In vitro biodegradation of wheat straw and wheat straw based total mixed ration using zoospores of different elite ruminal fungi
In vitro biodegradation of wheat straw mixed ration using zoospores of ruminal fungi
The elite anaerobic fungal cultures Neocallimastix sp GR-1, Piromyces sp WNG-12 and Orpinomyces sp C-14 were isolated from wild blue bull and cattle respectively of grazing and browsing goat. Attempts were made to grow these cultures in normal media or using stress conditions by keeping them at lower or higher temperature than 39oc or using a complex media; Normal media produced maximum number of fungal zoospores after 120 hrs of incubation. The in- vitro dry matter, acid detergent fibre, neutral detergent fibre digestibility were studied using fresh zoospores; or those being kept at normal; lower or higher temperature than 39oC. Based on in vitro digestibility of DM, NDF and ADF of wheat straw and wheat straw based total mixed rations. The Neocallimastix sp GR-1was found better as compared to Piromyces sp WNG-12 and Orpinomyces sp C-14as this anaerobic fungi produced maximum numbers of zoospores and also proved to be best performing for its ability to degrade lignified feed material.