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Chromosome8 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001033.1 1 SSR FORWARD 20 55.55 40 4 0 ACAAGGAGTTCCAATCACAA 264 4 1 CT 2 CTCTCTCTCTCTCTCT 16
CM001033.1 1 SSR REVERSE 20 55.46 40 4 2 GCCTCGACAAATGAAGATTA
CM001033.1 1 SSR FORWARD 20 55.55 40 3 1 AACTTGTCCCATCTTGTTGA 236 3 1 TG 2 TGTGTGTGTGTGTGTGTGTG 20
CM001033.1 1 SSR REVERSE 20 55.29 40 4 2 ATTTATATCCGTGCATGAGC
CM001033.1 1 SSR FORWARD 20 55.55 40 3 1 AACTTGTCCCATCTTGTTGA 236 3 1 TG 2 TGTGTGTGTGTGTGTGTGTG 20
CM001033.1 1 SSR REVERSE 20 55.29 40 4 2 ATTTATATCCGTGCATGAGC
CM001033.1 1 SSR FORWARD 20 55.18 45 6 1 ATCCAGGGTTCCCTATCTAA 240 4 2 AT 2 ATATATATATATATATAT 18
CM001033.1 1 SSR REVERSE 20 55.66 45 3 2 CCCTTATGTGGAGGAAGAAT
CM001033.1 1 SSR FORWARD 20 55.04 45 2 2 CTGTTTTTCCACCAAGTAGC 251 3 0 AC 2 ACACACACACACAC 14
CM001033.1 1 SSR REVERSE 20 55.11 45 6 2 ATAGGTATGCATCGATCGTC
CM001033.1 1 SSR FORWARD 20 55.53 40 5 2 AAGCATCATTGAACTTGCTC 239 3 0 CT 2 CTCTCTCTCTCT 12
CM001033.1 1 SSR REVERSE 20 55.49 45 4 2 TTTACTTCTACCGTGGTTGC
CM001033.1 1 SSR FORWARD 19 55.08 47.37 4 3 GACACGAACTCCCAAAGTT 279 3 1 TC 2 TCTCTCTCTCTCTCTCTCTCTC 22
CM001033.1 1 SSR REVERSE 19 55.31 47.37 8 2 CAATAGCATGCCACTGAAG
CM001033.1 1 SSR FORWARD 20 55.75 45 4 2 ACGTCGATTGAACACCTAGA 253 4 1 TC 2 TCTCTCTCTCTC 12
CM001033.1 1 SSR REVERSE 20 55.98 50 7 2 ACATACCTCAGGTTGACACG
CM001033.1 1 SSR FORWARD 20 56.05 50 4 2 GGACCTCTGCTTAGGGTTTA 264 4 3 TA 2 TATATATATATATA 14
CM001033.1 1 SSR REVERSE 22 55.4 40.91 3 1 GAACTCTCTGAACCTCTGAAAA
CM001033.1 1 SSR FORWARD 20 55.32 40 3 2 TTGAAACTTGAGCGAACTCT 252 3 1 CT 2 CTCTCTCTCTCT 12
CM001033.1 1 SSR REVERSE 20 55.12 50 2 0 TCACCACCACAACACTACAC
CM001033.1 1 SSR FORWARD 20 55.07 50 5 0 GTCCGACTGAGTGATCTTGT 264 5 3 AT 2 ATATATATATATATATAT 18
CM001033.1 1 SSR REVERSE 20 56.32 45 4 2 AACCGGGATTCAAGTCTCTA
CM001033.1 1 SSR FORWARD 19 55.15 52.63 6 0 CCCGGGTTGTAGAGATAGA 274 5 2 AT 2 ATATATATATATATATATATATAT 24
CM001033.1 1 SSR REVERSE 20 55.61 45 6 1 GTTCCAGGTACCCGTTTATT
CM001033.1 1 SSR FORWARD 20 55.42 50 6 0 TCCAGTTGGAGAGTGAAGAG 249 5 0 GA 2 GAGAGAGAGAGAGA 14
CM001033.1 1 SSR REVERSE 21 55.11 42.86 7 2 TTCACAGTCAGTGACACGATA
CM001033.1 1 SSR FORWARD 21 55.1 47.62 4 1 CCTACCCATGACTATGACTGA 251 4 2 AT 2 ATATATATATAT 12
CM001033.1 1 SSR REVERSE 20 55.43 45 3 0 TTGGCTATGTTAGAGGTTGG
CM001033.1 1 SSR FORWARD 20 55.29 45 3 1 CGCCTTGTAGGTGTATGATT 246 5 3 TA 2 TATATATATATATATATA 18
CM001033.1 1 SSR REVERSE 20 56.05 45 8 3 AGAATGTCGACAACATACCG
CM001033.1 1 SSR FORWARD 20 55.75 45 5 2 ACACAGACTACCGAATCGAA 233 3 2 AC 2 ACACACACACACAC 14
CM001033.1 1 SSR REVERSE 20 55.14 45 5 3 CGAGGAATAGAGGACCTTTT
CM001033.1 1 SSR FORWARD 20 55.03 45 2 0 TTCCTTTACCACCAGTCATC 271 3 2 AT 2 ATATATATATATAT 14
CM001033.1 1 SSR REVERSE 19 55.12 52.63 4 0 CACGGACAGTTACGTTGAG
CM001033.1 1 SSR FORWARD 20 55.03 45 2 0 TTCCTTTACCACCAGTCATC 271 3 2 TA 2 TATATATATATATATATATATATATATA 28
CM001033.1 1 SSR REVERSE 19 55.12 52.63 4 0 CACGGACAGTTACGTTGAG
CM001033.1 1 SSR FORWARD 20 55.75 45 5 2 ACACAGACTACCGAATCGAA 233 3 2 AC 2 ACACACACACACAC 14
CM001033.1 1 SSR REVERSE 20 55.14 45 5 3 CGAGGAATAGAGGACCTTTT
CM001033.1 1 SSR FORWARD 20 55.03 45 2 0 TTCCTTTACCACCAGTCATC 271 3 2 AT 2 ATATATATATATAT 14
CM001033.1 1 SSR REVERSE 19 55.12 52.63 4 0 CACGGACAGTTACGTTGAG
CM001033.1 1 SSR FORWARD 20 55.03 45 2 0 TTCCTTTACCACCAGTCATC 271 3 2 TA 2 TATATATATATATATATATATATATATA 28
CM001033.1 1 SSR REVERSE 19 55.12 52.63 4 0 CACGGACAGTTACGTTGAG
CM001033.1 1 SSR FORWARD 20 55.14 50 2 0 CTTCTCCAGCACTTTCTCAC 246 6 1 TC 2 TCTCTCTCTCTCTC 14
CM001033.1 1 SSR REVERSE 20 55.37 40 2 0 TTCTTTACTTGCTGGCTGTT
CM001033.1 1 SSR FORWARD 20 55.45 40 5 1 AGCTCAATTTCAAACACCAG 268 5 1 AT 2 ATATATATATAT 12
CM001033.1 1 SSR REVERSE 20 55.38 40 2 2 TCTAAACCGAACACGAAATC
CM001033.1 1 SSR FORWARD 21 56.37 42.86 3 0 CAGAAACATAGCCTCATCACA 267 3 1 AT 2 ATATATATATATATAT 16
CM001033.1 1 SSR REVERSE 20 55.22 40 4 2 CCGCAGTTATTCCACATATT
CM001033.1 1 SSR FORWARD 20 55.43 40 2 2 TAGGCACCAACCATTTTTAG 250 5 1 AG 2 AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG 32
CM001033.1 1 SSR REVERSE 20 55.19 40 3 0 AAACAATGCTCCAATCACTC
CM001033.1 1 SSR FORWARD 20 55.08 45 7 3 TCTTCTGTAACCGGAGAGAA 259 4 0 AG 2 AGAGAGAGAGAGAGAGAGAGAGAG 24
CM001033.1 1 SSR REVERSE 20 55.38 45 4 2 AATCAAACCCTAGAGGAGGA
CM001033.1 1 SSR FORWARD 20 55.09 45 2 0 AGAGGGAAAGGAAGAATGAG 258 4 1 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGA 28
CM001033.1 1 SSR REVERSE 21 55.07 47.62 4 3 AGTCCAAAGACTCCTGAAGAC
CM001033.1 1 SSR FORWARD 20 55.78 45 4 0 GCATGACCGCATATTAACTC 226 4 2 TC 2 TCTCTCTCTCTCTCTCTCTC 20
CM001033.1 1 SSR REVERSE 20 55.24 40 4 0 AGAAGAAGAAGAATGCACGA
CM001033.1 1 SSR FORWARD 20 55.78 45 4 0 GCATGACCGCATATTAACTC 226 4 2 TC 2 TCTCTCTCTCTC 12
CM001033.1 1 SSR REVERSE 20 55.24 40 4 0 AGAAGAAGAAGAATGCACGA
CM001033.1 1 SSR FORWARD 20 55.24 40 4 0 TCGTGCATTCTTCTTCTTCT 286 4 1 TA 2 TATATATATATA 12
CM001033.1 1 SSR REVERSE 20 55.78 45 3 1 GCACTAAAGCAGTTTCCTCA
CM001033.1 1 SSR FORWARD 20 55.16 40 5 0 TCTCTTGAAGCTGTTTTGGT 242 5 1 AT 2 ATATATATATATATAT 16
CM001033.1 1 SSR REVERSE 20 55.18 45 3 2 TAACCCTATCCAACGAGATG
CM001033.1 1 SSR FORWARD 21 55.51 42.86 6 0 TTGTCAGCAGATACGTAAACC 290 7 2 AT 2 ATATATATATATAT 14
CM001033.1 1 SSR REVERSE 20 55.51 40 4 0 CGACAATACGTTGTGATGAA
CM001033.1 1 SSR FORWARD 19 55.32 42.11 5 2 AGGAGGCCCAATTATTCTT 265 5 0 AT 2 ATATATATATAT 12
CM001033.1 1 SSR REVERSE 20 55.19 40 3 2 AGGAGCATCATTTTGTTGAC
CM001033.1 1 SSR FORWARD 19 55.32 42.11 5 2 AGGAGGCCCAATTATTCTT 265 5 0 AT 2 ATATATATATAT 12
CM001033.1 1 SSR REVERSE 20 55.19 40 3 2 AGGAGCATCATTTTGTTGAC
CM001033.1 1 SSR FORWARD 20 55.16 45 5 3 CGTGTTCAAGTAACCTGGAT 247 5 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 64
CM001033.1 1 SSR REVERSE 20 55.14 50 3 1 AGGAAGTATCAGGTCCCAGT
CM001033.1 1 SSR FORWARD 21 55.64 42.86 3 1 CTGAGTGATGTTTTTGTGGTC 243 5 1 CT 2 CTCTCTCTCTCT 12
CM001033.1 1 SSR REVERSE 20 55.37 45 2 0 GGTCTAACTCAAGCATTCCA
CM001033.1 1 SSR FORWARD 22 55.36 40.91 4 0 TCGATTTCAGTTCTCTAAGTCC 263 5 0 AT 2 ATATATATATATAT 14
CM001033.1 1 SSR REVERSE 20 56.05 40 6 2 CCAAAACGATCGATATACCA
CM001033.1 1 SSR FORWARD 20 55.35 45 3 1 GAGTTGTTCTTTGGCAGTGT 238 4 3 GT 2 GTGTGTGTGTGTGT 14
CM001033.1 1 SSR REVERSE 20 55.28 55 2 0 CCCAGACCTACACATACACC
CM001033.1 1 SSR FORWARD 20 55.49 50 4 0 AGAGTACGCAGATGGTGAAG 249 6 0 CT 2 CTCTCTCTCTCTCT 14
CM001033.1 1 SSR REVERSE 20 55.36 45 6 2 AAGAGATCTGCAATGGAGTG
CM001033.1 1 SSR FORWARD 20 55.07 45 4 2 ACGTGGAAGGATTCATAGTG 258 4 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 54
CM001033.1 1 SSR REVERSE 20 55.36 50 6 0 TCACAGAGCTCCCTTGATAC
CM001033.1 1 SSR FORWARD 20 55.25 40 3 0 ATTGCTTGTTGGAATGTAGG 246 4 2 AG 2 AGAGAGAGAGAG 12
CM001033.1 1 SSR REVERSE 20 55.24 40 6 2 TTCGAAAGTCTTCTGCTCAT
CM001033.1 1 SSR FORWARD 20 55.03 45 5 2 GACTGGTCACCCAGAAATTA 253 3 1 GA 2 GAGAGAGAGAGAGAGAGA 18
CM001033.1 1 SSR REVERSE 20 55.82 45 2 0 AGGAAGAAGATAACGGGATG
CM001033.1 1 SSR FORWARD 20 55.76 40 3 3 TTATCCAAGCCCTGATTGTA 214 4 1 AT 2 ATATATATATATATATAT 18
CM001033.1 1 SSR REVERSE 20 56.19 45 6 2 TGAGGCTAGCAGCACATATT
CM001033.1 1 SSR FORWARD 19 55.54 47.37 4 0 TGAACTCCCTAGCCAAAAG 263 4 1 TC 2 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC 46
CM001033.1 1 SSR REVERSE 20 55.13 55 2 2 AGGAGAGAAGAGGAGACGAC
CM001033.1 1 SSR FORWARD 20 55.13 55 2 0 GTCGTCTCCTCTTCTCTCCT 245 2 1 TC 2 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC 40
CM001033.1 1 SSR REVERSE 20 55.16 40 2 2 GTCCATTTTCGTCATAGCAT
CM001033.1 1 SSR FORWARD 20 55.36 45 4 2 CATGGACTGCTGAAGAAGAT 264 2 2 CA 2 CACACACACACACACACACACACA 24
CM001033.1 1 SSR REVERSE 19 56.17 47.37 6 2 TGTGTACCCCGGTGTTAAT
CM001033.1 1 SSR FORWARD 20 55.08 50 3 2 ATCGGAGGTAGAGATTAGGC 260 4 1 AG 2 AGAGAGAGAGAGAGAG 16
CM001033.1 1 SSR REVERSE 20 55.29 40 3 1 AGACTCCAAATAGCAGCAAA
CM001033.1 1 SSR FORWARD 20 55.28 50 3 2 CAATGTCTCAGCCTCTAAGC 251 2 0 TA 2 TATATATATATA 12
CM001033.1 1 SSR REVERSE 19 56.17 42.11 4 2 TGCAAATGGGTATGGGTAT
CM001033.1 1 SSR FORWARD 20 56.21 50 4 3 TCTAAGCCCACTCATGTGAC 243 5 3 TA 2 TATATATATATA 12
CM001033.1 1 SSR REVERSE 20 57.87 40 8 0 GTTTTTGCAAATGGGTATGG
CM001033.1 1 SSR FORWARD 20 55.14 50 4 0 CTATCCAGAAGGACGTGTGT 246 4 0 TA 2 TATATATATATA 12
CM001033.1 1 SSR REVERSE 20 55.64 40 4 2 GGGCGGTTATATTTTGGTAT
CM001033.1 1 SSR FORWARD 18 57.33 44.44 2 0 AAAACCAAAACCCCGAAC 275 4 0 TA 2 TATATATATATATA 14
CM001033.1 1 SSR REVERSE 20 55.58 45 5 0 GGAGAGCTTTGACACAGAAA
CM001033.1 1 SSR FORWARD 20 55 45 7 3 CAAGAAGGTTCAGAACTTGG 243 5 1 CA 2 CACACACACACACA 14
CM001033.1 1 SSR REVERSE 20 55.05 40 3 2 GTGTGTTTGGGGATTCTAAA
CM001033.1 1 SSR FORWARD 20 55 55 4 0 GGTTGAGCACAGAGTAGGAG 265 7 3 AG 2 AGAGAGAGAGAGAGAGAGAGAG 22
CM001033.1 1 SSR REVERSE 20 55.04 40 4 2 AAAGCAAACCTCTGTACCAA
CM001033.1 1 SSR FORWARD 19 55.09 57.89 4 3 CAGCCCTGACTCTAAGGAG 246 3 3 GA 2 GAGAGAGAGAGA 12
CM001033.1 1 SSR REVERSE 20 55.57 50 5 3 CCACTCGAACTGTTACTGCT

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