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Chromosome7 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001032.1 1 SSR REVERSE 20 56.14 40 4 0 TTGATGACCTTTAGGGGTTT
CM001032.1 1 SSR FORWARD 20 55.22 45 6 2 CCGACGAACTTGAGTTTTAG 285 3 0 TA 2 TATATATATATATATATATATA 22
CM001032.1 1 SSR REVERSE 20 56.14 40 4 0 TTGATGACCTTTAGGGGTTT
CM001032.1 1 SSR FORWARD 20 55.29 45 4 0 GTCCTATTCTCATGCTCCAA 247 5 3 AC 2 ACACACACACAC 12
CM001032.1 1 SSR REVERSE 20 55.01 45 6 1 CAATGGAGATCTTGTGGAGT
CM001032.1 1 SSR FORWARD 20 55.06 45 2 1 GATGGTGGAGAAGAAGATGA 271 5 1 AT 2 ATATATATATATAT 14
CM001032.1 1 SSR REVERSE 20 55.08 45 6 1 GATCTTTCGAGAAGGGAACT
CM001032.1 1 SSR FORWARD 20 55.05 45 4 2 CTTATGATGGAAGCCTCAAG 252 5 0 AT 2 ATATATATATATATATATATATATATAT 28
CM001032.1 1 SSR REVERSE 20 55.29 40 7 2 CAGTGTCCAATGAACATCAA
CM001032.1 1 SSR FORWARD 20 55.06 45 5 2 GGATGATCTGGAAGTGAAGA 244 5 0 TC 2 TCTCTCTCTCTCTCTC 16
CM001032.1 1 SSR REVERSE 20 55.62 45 3 0 GGCAGACTAACAGAAACGAA
CM001032.1 1 SSR FORWARD 19 55.32 42.11 4 1 CGGTACGAAAAACCAAAGT 252 3 1 TA 2 TATATATATATA 12
CM001032.1 1 SSR REVERSE 20 55.42 40 3 2 GCACCAAACGAGGTAAAATA
CM001032.1 1 SSR FORWARD 20 55.04 45 3 0 GTTGTCTACCGACGAAAAAG 250 3 1 AT 2 ATATATATATATAT 14
CM001032.1 1 SSR REVERSE 19 55.68 47.37 6 2 AATCTGCTCGAAGCTGAAC
CM001032.1 1 SSR FORWARD 20 55.49 45 4 2 AAATGCCTGTACGAAGAGTG 226 2 0 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 76
CM001032.1 1 SSR REVERSE 20 55.58 40 4 2 TTAACGGTGTGGAGTTGAAT
CM001032.1 1 SSR FORWARD 20 55.77 50 4 1 TCAGCTCTCTCAAAGGTCAG 250 4 2 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 44
CM001032.1 1 SSR REVERSE 18 55.18 44.44 2 2 TAATCGCTGGTTTCTGGA
CM001032.1 1 SSR FORWARD 20 55.77 50 4 1 TCAGCTCTCTCAAAGGTCAG 254 4 0 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 34
CM001032.1 1 SSR REVERSE 20 55.09 40 3 1 ACACGACCCATTATTCAAAG
CM001032.1 1 SSR FORWARD 20 55.19 45 4 2 TTGGTGACTAGAGCACATGA 270 4 3 AT 2 ATATATATATATATATATATAT 22
CM001032.1 1 SSR REVERSE 20 55.02 40 4 2 CCAAATTCTCTTTCATCTCG
CM001032.1 1 SSR FORWARD 20 55.42 40 4 3 TCATCTTGCTTTCCTGAATC 250 3 1 GA 2 GAGAGAGAGAGA 12
CM001032.1 1 SSR REVERSE 20 55.6 40 4 2 TGGTGTTGTTGTTGTCATGT
CM001032.1 1 SSR FORWARD 21 55.12 42.86 2 0 ATGAGTGAGAGTAATGCGAGA 292 2 0 GA 2 GAGAGAGAGAGAGAGAGA 18
CM001032.1 1 SSR REVERSE 20 55 45 3 2 ACAGTGGTGAGTTTTGAACC
CM001032.1 1 SSR FORWARD 20 55.02 40 4 1 TGTACCTGTCGATGTTTTGA 252 6 0 AG 2 AGAGAGAGAGAGAGAGAG 18
CM001032.1 1 SSR REVERSE 20 55.59 45 5 1 TCAGCAATAGGTAACCGAAG
CM001032.1 1 SSR FORWARD 20 55.29 45 4 2 TCCTGAGCCTGAAATCATAC 252 5 3 TA 2 TATATATATATA 12
CM001032.1 1 SSR REVERSE 20 55.37 40 3 2 CAAAGAATCCAACTCAGCAT
CM001032.1 1 SSR FORWARD 20 55.12 40 3 2 ACTTTGAGGCAGAAATCGTA 238 4 2 AT 2 ATATATATATATATATAT 18
CM001032.1 1 SSR REVERSE 20 55.48 45 2 2 AAGCAAACAGAGGGGACTAT
CM001032.1 1 SSR FORWARD 20 55.04 50 4 1 CGGTTGAGAGAACCTACTTG 255 5 2 CT 2 CTCTCTCTCTCT 12
CM001032.1 1 SSR REVERSE 21 55.04 42.86 6 1 CCTCACCGGTATAATCTTTCT
CM001032.1 1 SSR FORWARD 20 55.67 45 5 1 TAACATCTCACATGCCCTCT 247 2 1 AT 2 ATATATATATATATATATATATATATATATATATAT 36
CM001032.1 1 SSR REVERSE 20 55.24 50 4 0 TCTAGCCTCTCACTCTGGAA
CM001032.1 1 SSR FORWARD 20 56.05 40 5 0 AGACATTGACTCGCTCAAAA 248 4 0 TC 2 TCTCTCTCTCTCTCTCTCTCTCTCTCTC 28
CM001032.1 1 SSR REVERSE 20 55.22 45 2 0 CTGGTAAACTGGAGAATGGA
CM001032.1 1 SSR FORWARD 19 55.01 42.11 7 2 GTTTCCCTGTGAAAACGAT 250 5 3 TC 2 TCTCTCTCTCTC 12
CM001032.1 1 SSR REVERSE 20 55.43 40 5 3 TCTTCTTCTTCATCGGATTG
CM001032.1 1 SSR FORWARD 20 55.61 40 2 2 AAGAAGAGAAAGCCACCAAT 254 4 1 AG 2 AGAGAGAGAGAG 12
CM001032.1 1 SSR REVERSE 19 55.08 47.37 7 3 TCTCCATCGTCACGAACTA
CM001032.1 1 SSR FORWARD 20 55.04 45 4 2 GGTTTGTTTCACTAGCAAGG 249 6 0 GA 2 GAGAGAGAGAGAGAGAGAGAGA 22
CM001032.1 1 SSR REVERSE 20 55.17 40 2 0 GAACACAAAAAGAGGCAAAG
CM001032.1 1 SSR FORWARD 20 55.33 45 5 2 GCCCTACAGAATCGTATGAA 264 6 3 AG 2 AGAGAGAGAGAGAGAGAGAGAGAG 24
CM001032.1 1 SSR REVERSE 20 55.13 40 4 0 TCATTTCGTAGGACCATTTC
CM001032.1 1 SSR FORWARD 20 55.37 45 8 3 TAGCAGCTCTGCTTTTAAGG 240 3 0 AT 2 ATATATATATAT 12
CM001032.1 1 SSR REVERSE 20 56.71 40 4 0 GTTTCGCGGGTATTTATTGT
CM001032.1 1 SSR FORWARD 20 55.56 40 4 0 TATGGGAAAATCTGGAGACA 247 5 1 AC 2 ACACACACACAC 12
CM001032.1 1 SSR REVERSE 19 55.25 42.11 3 0 AGAGATTCGTTGCTTGCTT
CM001032.1 1 SSR FORWARD 20 55.4 50 5 1 ACGAAACTCGTACTGACCTG 251 3 0 CT 2 CTCTCTCTCTCTCTCTCTCT 20
CM001032.1 1 SSR REVERSE 20 55.14 45 3 2 CGAAACCTCTCCCTCTTTAT
CM001032.1 1 SSR FORWARD 20 55.69 45 7 3 GAGTTGCAGAGTTGCAAAGT 253 4 1 TA 2 TATATATATATATA 14
CM001032.1 1 SSR REVERSE 20 55.05 45 2 2 TAAGGAACTGGAGAGGCATA
CM001032.1 1 SSR FORWARD 20 55.3 45 6 0 CAGAGGGTAAACCACTCAAA 292 5 3 TA 2 TATATATATATATATA 16
CM001032.1 1 SSR REVERSE 21 55.23 42.86 4 0 CCAGATTCGGTCTAACTCTTT
CM001032.1 1 SSR FORWARD 20 55.98 45 6 0 CGCGGATATTTATCGTAGTG 284 4 1 TA 2 TATATATATATATATATATA 20
CM001032.1 1 SSR REVERSE 20 55.62 40 2 2 CAGCAGAAAAACGAAGTCAT
CM001032.1 1 SSR FORWARD 19 55.32 42.11 4 2 TAGCTTATGGGCAACACAA 250 4 1 AT 2 ATATATATATAT 12
CM001032.1 1 SSR REVERSE 19 55.49 42.11 4 2 TGTCCCACCGTTTCTTAAT
CM001032.1 1 SSR FORWARD 20 55.66 45 4 2 CAGGGAAAGTACAAAGCGTA 251 4 2 AT 2 ATATATATATATATATATATAT 22
CM001032.1 1 SSR REVERSE 21 55.08 42.86 7 3 AACCAGTGGTGACAATGTAAG
CM001032.1 1 SSR FORWARD 20 55.15 40 3 1 CTCAAACCCAATGATAAGGA 250 4 0 AG 2 AGAGAGAGAGAG 12
CM001032.1 1 SSR REVERSE 20 55.19 45 4 1 ACCAAGACAAGTGGTTGAAG
CM001032.1 1 SSR FORWARD 19 55.19 42.11 3 0 GGAAATGGGTGTCAAAATC 265 4 2 TA 2 TATATATATATATATA 16
CM001032.1 1 SSR REVERSE 21 55.2 42.86 3 2 ACTGTTAGACTTGAGCGATGA
CM001032.1 1 SSR FORWARD 19 55.19 42.11 3 0 GGAAATGGGTGTCAAAATC 265 4 2 TA 2 TATATATATATATATA 16
CM001032.1 1 SSR REVERSE 21 55.2 42.86 3 2 ACTGTTAGACTTGAGCGATGA
CM001032.1 1 SSR FORWARD 20 55.42 40 4 2 TCATTGGGCTTAATATCTGC 245 4 2 GA 2 GAGAGAGAGAGA 12
CM001032.1 1 SSR REVERSE 20 55.38 45 3 2 TATGACCCAGAAGAAAGTCG
CM001032.1 1 SSR FORWARD 20 55.34 40 3 2 AAACCCTAATGACACCATCA 255 4 1 CA 2 CACACACACACACACA 16
CM001032.1 1 SSR REVERSE 20 55.12 40 6 2 ATCCTGGAAAGATCTCCATT
CM001032.1 1 SSR FORWARD 20 55.7 40 3 0 AACGAAGCACTGAAACAAAG 259 4 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 62
CM001032.1 1 SSR REVERSE 20 55.08 50 6 3 CTAGGACCCTACCATTGTCA
CM001032.1 1 SSR FORWARD 20 55.22 45 4 1 CTAGAACCCATTGACTTGGA 256 3 1 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 56
CM001032.1 1 SSR REVERSE 19 55.11 52.63 4 0 GTTTGCCACCTGTACCTCT
CM001032.1 1 SSR FORWARD 20 55.06 45 4 3 GCCTTCCATTACTGTGTCAT 251 2 0 CT 2 CTCTCTCTCTCTCT 14
CM001032.1 1 SSR REVERSE 20 55.5 50 2 0 CCTCTTCCCATCTTTCTCTC
CM001032.1 1 SSR FORWARD 20 55.08 40 4 0 GACTGATCTTTGGAAATGGA 252 4 0 GA 2 GAGAGAGAGAGA 12
CM001032.1 1 SSR REVERSE 22 55.2 40.91 4 3 CTAACCCCCGTTATTAGTGTAA
CM001032.1 1 SSR FORWARD 20 55.08 40 6 2 TGCCACAAGTTAACACCATA 254 3 1 AG 2 AGAGAGAGAGAGAGAG 16
CM001032.1 1 SSR REVERSE 20 55.93 55 5 2 GTCATCCCTGAGAGGTATCC
CM001032.1 1 SSR FORWARD 20 55.31 40 5 3 CGAGAAAAGCTACAATTTGG 262 5 1 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGA 26
CM001032.1 1 SSR REVERSE 20 55.09 40 3 1 AGCAGCCATTAGGTATTTCA
CM001032.1 1 SSR FORWARD 20 55.54 45 4 0 TATGACAGACGCTTTCTTCC 268 6 2 GA 2 GAGAGAGAGAGAGA 14
CM001032.1 1 SSR REVERSE 20 55.5 40 2 0 AGGAAGAGAAAAAGCAAAGG
CM001032.1 1 SSR FORWARD 20 55.96 40 7 2 TACCCCGTTATGGGTTAAAT 259 5 0 CT 2 CTCTCTCTCTCTCT 14
CM001032.1 1 SSR REVERSE 20 56.43 40 4 0 CCCATGAAAAGGAAAGAAAG
CM001032.1 1 SSR FORWARD 20 55.6 40 2 0 CACGAAAAGAAGAGGTCAAA 247 4 0 TC 2 TCTCTCTCTCTCTC 14
CM001032.1 1 SSR REVERSE 20 55.67 55 7 1 GAGAGATTCTCGGAGAGAGG
CM001032.1 1 SSR FORWARD 20 55.5 45 5 2 TTAACGCTTAGCCTGACCTA 251 3 1 TG 2 TGTGTGTGTGTGTGTGTG 18
CM001032.1 1 SSR REVERSE 20 55.23 45 6 2 GACGCCACAAATATCTGACT
CM001032.1 1 SSR FORWARD 20 55.14 45 4 1 GTGGATCTGAGCGATACATT 250 5 2 TC 2 TCTCTCTCTCTCTCTCTC 18
CM001032.1 1 SSR REVERSE 20 55.11 40 4 2 TGTGAATGATCCTGTAGCAA
CM001032.1 1 SSR FORWARD 20 55.1 50 2 1 CTCTCTCTCTCTCGCTTTCA 234 5 3 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 32
CM001032.1 1 SSR REVERSE 20 56.02 40 4 2 AGGAATTAGCCGAAAAGCTA
CM001032.1 1 SSR FORWARD 20 55.6 40 6 2 TATAACCGTGGAGATCCAAA 240 5 2 AT 2 ATATATATATATATATAT 18
CM001032.1 1 SSR REVERSE 20 55.38 45 5 2 TAAGATTCGACGAGCCTAGA
CM001032.1 1 SSR FORWARD 22 55.39 40.91 4 0 GACACTTATCACTTTCGACACA 259 3 2 TA 2 TATATATATATATATA 16
CM001032.1 1 SSR REVERSE 20 56.98 40 4 2 CCACGTCCACATTTTGTTTA
CM001032.1 1 SSR FORWARD 21 55.71 52.38 3 2 CCCACTCACATAGTCCCTACT 248 5 2 AT 2 ATATATATATAT 12
CM001032.1 1 SSR REVERSE 20 56.17 45 2 2 AGGTGAGGGTTTATTCCGTA
CM001032.1 1 SSR FORWARD 19 55.78 42.11 2 0 CCGTTGATTTGATTGTGTG 254 4 1 AT 2 ATATATATATAT 12
CM001032.1 1 SSR REVERSE 20 55.76 55 7 2 ACTGCGTCGCTAGTACAGTC
CM001032.1 1 SSR FORWARD 20 55.17 40 3 1 TTAGCCCTGCTGTGTTTATT 247 4 1 TA 2 TATATATATATA 12
CM001032.1 1 SSR REVERSE 20 55.09 45 6 2 TGAGATGCATACAGTCCAGA
CM001032.1 1 SSR FORWARD 20 55.5 40 6 3 GAGAACAAATCTTTGCCAGA 247 4 0 GA 2 GAGAGAGAGAGA 12
CM001032.1 1 SSR REVERSE 20 55.12 45 5 2 GGACTCGGATTCACTTGATA
CM001032.1 1 SSR FORWARD 20 55.19 50 7 1 TCCACTGGACTCGATACTTC 248 4 2 AT 2 ATATATATATAT 12
CM001032.1 1 SSR REVERSE 20 55.77 45 6 0 CCCATGCATCAAGATAAGAG
CM001032.1 1 SSR FORWARD 20 55.36 45 3 0 AGGAGTGGACCTTTGAGTTT 272 3 1 TC 2 TCTCTCTCTCTCTCTC 16
CM001032.1 1 SSR REVERSE 20 55.64 50 3 0 AGGAGACGAAGCACCTATCT
CM001032.1 1 SSR FORWARD 20 55.18 40 4 2 TTCGTCTCCTTCTTCGATAA 249 5 2 TC 2 TCTCTCTCTCTCTCTCTC 18
CM001032.1 1 SSR REVERSE 20 55.95 40 6 3 TTCGAAATCTCCAAGTCAGA
CM001032.1 1 SSR FORWARD 20 55.48 50 2 2 AAAGACGGAGAAGAGAGAGC 257 3 0 TC 2 TCTCTCTCTCTCTCTC 16
CM001032.1 1 SSR REVERSE 20 55.47 40 2 0 CTAAAACTGAACCGAACCAA
CM001032.1 1 SSR FORWARD 20 56.34 40 6 2 CGTCAAAACGTTAATCGGTA 271 4 2 AT 2 ATATATATATATATATATATATATATATAT 30
CM001032.1 1 SSR REVERSE 20 55.15 40 5 3 TTTTCTACACAGGCCCTTTA
CM001032.1 1 SSR FORWARD 20 55.5 40 4 2 TTGAAAATCCAAGCTCTGAC 252 4 2 AG 2 AGAGAGAGAGAGAG 14
CM001032.1 1 SSR REVERSE 20 55.17 40 4 0 AGTTGTTATGTTCCGGTTTG
CM001032.1 1 SSR FORWARD 20 55.33 50 3 2 CCTGGGTTCACATCTCATAC 251 3 1 TA 2 TATATATATATA 12
CM001032.1 1 SSR REVERSE 20 55.34 40 3 2 GCAAATCAACGGGATACTTA
CM001032.1 1 SSR FORWARD 21 55.23 42.86 2 0 TCTTCATCCTCCTCTTTCTTC 242 5 3 TC 2 TCTCTCTCTCTCTCTCTCTCTCTC 24
CM001032.1 1 SSR REVERSE 19 55.17 52.63 2 0 GGGAAGATTAGGGTTAGGG

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