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Chromosome6 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001031.1 1 SSR FORWARD 20 55.12 40 4 2 CATTTCAAGGGTGAACTTGT 267 4 3 TC 2 TCTCTCTCTCTCTCTCTCTC 20
CM001031.1 1 SSR REVERSE 20 55.89 40 4 2 GATGGCCGAGAAAATAAGAT
CM001031.1 1 SSR FORWARD 20 55.67 45 3 2 ACCTCAGCATTGGTATCTCA 241 3 1 TC 2 TCTCTCTCTCTCTCTCTCTC 20
CM001031.1 1 SSR REVERSE 19 55.25 47.37 6 3 GAGTTTTCCGACGTCACTT
CM001031.1 1 SSR FORWARD 20 55.06 40 6 2 CGAAATTTTGGTATCAGAGC 248 3 0 AT 2 ATATATATATATATATATATATATATATATATAT 34
CM001031.1 1 SSR REVERSE 20 55.37 45 4 1 TTAGTCGAAGGACGAATGAC
CM001031.1 1 SSR FORWARD 20 56.65 40 4 2 GATTTGCGGGAAGATGTTAT 266 4 1 TA 2 TATATATATATATATATA 18
CM001031.1 1 SSR REVERSE 21 55.83 47.62 5 3 GGAAACCACCTTAGGAGTGTA
CM001031.1 1 SSR FORWARD 20 55.06 40 6 2 CGAAATTTTGGTATCAGAGC 252 3 0 AT 2 ATATATATATATATATATATATATATATATATAT 34
CM001031.1 1 SSR REVERSE 20 55.37 45 4 1 TTAGTCGAAGGACGAATGAC
CM001031.1 1 SSR FORWARD 20 55.06 40 6 2 CGAAATTTTGGTATCAGAGC 248 3 0 AT 2 ATATATATATATATATATATATATATATATATAT 34
CM001031.1 1 SSR REVERSE 20 55.37 45 4 1 TTAGTCGAAGGACGAATGAC
CM001031.1 1 SSR FORWARD 20 55.67 40 4 1 ACGGATAACAAATCGAGTCA 274 4 0 AT 2 ATATATATATATATATATAT 20
CM001031.1 1 SSR REVERSE 20 55.31 45 6 2 AATAGACACGTGGCAACTTC
CM001031.1 1 SSR FORWARD 20 55.01 45 2 0 AAGAGTGATGTGGAAGGATG 257 4 1 AT 2 ATATATATATATAT 14
CM001031.1 1 SSR REVERSE 20 55.65 40 3 3 TCTTATGTGGGAGGTTTCAA
CM001031.1 1 SSR FORWARD 22 55.12 45.45 2 1 AGTGAGGTAGGATAGGAGGATT 284 3 1 AT 2 ATATATATATATATAT 16
CM001031.1 1 SSR REVERSE 20 55.3 45 4 2 GGTAGTATTGCCGGTTGTTA
CM001031.1 1 SSR FORWARD 20 55.02 50 4 0 CAGCTCTGTCACTTTTACCC 246 5 2 TC 2 TCTCTCTCTCTC 12
CM001031.1 1 SSR REVERSE 20 55.37 45 4 2 TTAACAACTCAGGGAGCATC
CM001031.1 1 SSR FORWARD 20 55.01 45 4 0 AGGTGGATCATGTGAAGAAG 252 4 1 CT 2 CTCTCTCTCTCTCT 14
CM001031.1 1 SSR REVERSE 20 55.67 40 5 2 CGTATTTGGAATGACGAAAC
CM001031.1 1 SSR FORWARD 20 55.32 45 4 2 AGGAAGATTTGTCGGGTACT 247 3 3 TA 2 TATATATATATATA 14
CM001031.1 1 SSR REVERSE 20 55 55 5 1 CACCTTAGCACTCAGTCTCC
CM001031.1 1 SSR FORWARD 20 55.91 45 3 1 CGTATTTCGTCTCGTTTGAC 249 3 2 TC 2 TCTCTCTCTCTCTC 14
CM001031.1 1 SSR REVERSE 20 55.2 40 3 0 TGTATTTCACTGCCTCACAA
CM001031.1 1 SSR FORWARD 20 55.66 40 7 3 ACTTTTGGTTACCACACGAA 238 2 0 TA 2 TATATATATATATA 14
CM001031.1 1 SSR REVERSE 20 56.1 60 2 0 GCGGAGAGAGAGAGAGAGAG
CM001031.1 1 SSR FORWARD 20 55.46 40 2 0 TATTCATCACTTCGCTTTCC 263 2 0 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 34
CM001031.1 1 SSR REVERSE 20 55.18 45 3 0 ACTTCTATTACCGCCTTTCC
CM001031.1 1 SSR FORWARD 20 55.92 55 3 0 CTTTCCAGCCTCTCTCTCTC 249 2 0 TC 2 TCTCTCTCTCTCTC 14
CM001031.1 1 SSR REVERSE 20 55.18 45 3 0 ACTTCTATTACCGCCTTTCC
CM001031.1 1 SSR FORWARD 20 55.15 45 4 1 CATCCTGATCTTGGCATAGT 249 5 2 TA 2 TATATATATATA 12
CM001031.1 1 SSR REVERSE 19 55.4 52.63 3 0 GGGCTACGACCAGACTAAA
CM001031.1 1 SSR FORWARD 20 55.15 45 4 1 CATCCTGATCTTGGCATAGT 249 5 2 TA 2 TATATATATATATATATATATA 22
CM001031.1 1 SSR REVERSE 19 55.4 52.63 3 0 GGGCTACGACCAGACTAAA
CM001031.1 1 SSR FORWARD 19 55.75 47.37 5 3 CCTCTGGCAAATTGATAGG 261 4 2 TA 2 TATATATATATATATA 16
CM001031.1 1 SSR REVERSE 20 55.58 40 3 2 TTTCTCAAACCTCAACAAGC
CM001031.1 1 SSR FORWARD 20 56.34 45 5 2 TCATTCTTGCTGACAAGTCC 238 5 0 AT 2 ATATATATATAT 12
CM001031.1 1 SSR REVERSE 20 55.5 45 7 3 TCCAAGATTCTGAAGTCTGC
CM001031.1 1 SSR FORWARD 20 56.15 45 3 2 TTGGCGATTGAGAGAGACTA 234 4 1 AG 2 AGAGAGAGAGAGAGAGAGAG 20
CM001031.1 1 SSR REVERSE 20 55.41 45 4 1 ATCATCTACGTCCAAAGCAG
CM001031.1 1 SSR FORWARD 20 55.16 40 8 2 TCATATGATGTTCCACGATG 242 5 1 AG 2 AGAGAGAGAGAGAGAGAGAG 20
CM001031.1 1 SSR REVERSE 20 55.41 45 4 1 ATCATCTACGTCCAAAGCAG
CM001031.1 1 SSR FORWARD 20 55.2 45 3 0 GGAGAACTTGTGGATTACGA 247 2 0 AT 2 ATATATATATATAT 14
CM001031.1 1 SSR REVERSE 20 55.79 50 4 0 GCTGCTGATATGGGACTATG
CM001031.1 1 SSR FORWARD 20 55.31 40 4 2 TATCCCTTGGAAGATCCTTT 231 4 1 TG 2 TGTGTGTGTGTGTGTGTGTGTG 22
CM001031.1 1 SSR REVERSE 20 55.07 40 2 2 TATTCCTTTGGTCTTGCCTA
CM001031.1 1 SSR FORWARD 21 55.58 42.86 6 0 CTCGAGTTCGAAACTTCTCTT 263 4 3 AG 2 AGAGAGAGAGAGAG 14
CM001031.1 1 SSR REVERSE 20 55.2 40 2 0 TGGAGCAAAGACACATTACA
CM001031.1 1 SSR FORWARD 20 55.07 40 4 2 ACAGAAAGAGGATTTGCTCA 295 4 2 TA 2 TATATATATATA 12
CM001031.1 1 SSR REVERSE 19 55.23 42.11 5 2 TGCTTGATTCAGAGGAACA
CM001031.1 1 SSR FORWARD 20 56.22 45 4 2 GATGTATCACCCCCGTTAAT 220 3 0 CA 2 CACACACACACACACACA 18
CM001031.1 1 SSR REVERSE 20 57.92 40 4 0 CAATCCACCGATTTTTGTTC
CM001031.1 1 SSR FORWARD 20 55 45 2 2 CCCTTGTCTTGTTTCTTCAG 245 4 0 GT 2 GTGTGTGTGTGTGTGT 16
CM001031.1 1 SSR REVERSE 20 55.44 40 7 3 ACTGCCATAACATTGTTGGT
CM001031.1 1 SSR FORWARD 20 55.11 45 4 2 GTTTGAGCCACTTCATAAGC 257 4 0 CT 2 CTCTCTCTCTCTCTCTCTCTCTCT 24
CM001031.1 1 SSR REVERSE 21 55.95 42.86 5 2 AAACATGGAGACCATACCTGT
CM001031.1 1 SSR FORWARD 20 56.1 40 4 0 GACAAAAATTCCCCTCAAAC 244 4 3 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCT 26
CM001031.1 1 SSR REVERSE 20 55.49 45 4 2 ATTGTGCCTTTCAGTACGAG
CM001031.1 1 SSR FORWARD 20 55.1 45 4 0 ACTATTAACGCTGGTGCTGT 267 2 0 CT 2 CTCTCTCTCTCT 12
CM001031.1 1 SSR REVERSE 20 55.2 50 0 0 TCTCCTCTTCCTTCCTCTTC
CM001031.1 1 SSR FORWARD 20 55.24 45 6 2 TGTTGGTTGGTGTACATGAG 241 4 1 TC 2 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC 62
CM001031.1 1 SSR REVERSE 20 55.91 40 2 0 TTTCCATCCTTCCTTCTTTC
CM001031.1 1 SSR FORWARD 20 55.3 45 5 0 TCAGGTCTGTACCTTTTTGG 271 5 2 AT 2 ATATATATATATAT 14
CM001031.1 1 SSR REVERSE 20 55.39 40 7 2 CGATCTGAAATCGAATAGGA
CM001031.1 1 SSR FORWARD 20 55.19 45 2 0 ATGACGAGAGGAGTTGATTG 267 6 3 GT 2 GTGTGTGTGTGT 12
CM001031.1 1 SSR REVERSE 20 55.3 45 4 3 CGAACATGGAGATTCCTCTA
CM001031.1 1 SSR FORWARD 20 56.61 55 4 2 ACGCTAGAGAGACAGGGGTA 242 3 1 TA 2 TATATATATATA 12
CM001031.1 1 SSR REVERSE 20 55.36 40 4 0 AAAACTTCTGCAACCACAAC
CM001031.1 1 SSR FORWARD 20 55.41 45 5 1 ATAGGGGACAGTGTTGTTGA 250 5 2 TA 2 TATATATATATA 12
CM001031.1 1 SSR REVERSE 20 55.19 45 3 2 TCTCAGCAAGTGTGGTCATA
CM001031.1 1 SSR FORWARD 20 55.23 45 6 2 TGGGGCTTAGTATACATTGG 244 3 0 TC 2 TCTCTCTCTCTCTC 14
CM001031.1 1 SSR REVERSE 19 55.34 57.89 4 0 GGCGCATAGAGAGAGAGAG
CM001031.1 1 SSR FORWARD 20 55.23 45 6 2 TGGGGCTTAGTATACATTGG 244 3 0 CT 2 CTCTCTCTCTCTCT 14
CM001031.1 1 SSR REVERSE 19 55.34 57.89 4 0 GGCGCATAGAGAGAGAGAG
CM001031.1 1 SSR FORWARD 20 55.23 45 6 2 TGGGGCTTAGTATACATTGG 300 3 0 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCT 28
CM001031.1 1 SSR REVERSE 21 55.19 57.14 2 0 GAGAGAGAGCAGAGAGAGAGG
CM001031.1 1 SSR FORWARD 18 56.09 55.56 4 0 TCTCTCTATGCGCCCTCT 234 7 3 CT 2 CTCTCTCTCTCT 12
CM001031.1 1 SSR REVERSE 20 57.97 60 1 0 GGGAGAGAGAGAGTGGGAAG
CM001031.1 1 SSR FORWARD 18 56.09 55.56 4 0 TCTCTCTATGCGCCCTCT 234 7 3 CT 2 CTCTCTCTCTCTCTCTCT 18
CM001031.1 1 SSR REVERSE 20 57.97 60 1 0 GGGAGAGAGAGAGTGGGAAG
CM001031.1 1 SSR FORWARD 20 56.1 60 2 0 CCGCTCTCTCTCTCTCTCTC 297 3 0 CT 2 CTCTCTCTCTCTCT 14
CM001031.1 1 SSR REVERSE 20 55.17 40 5 3 TCGAGGAAATCATCTCAATC
CM001031.1 1 SSR FORWARD 20 55.14 55 1 0 CTCTCTCCCCACTCTCTCTT 238 3 0 CT 2 CTCTCTCTCTCT 12
CM001031.1 1 SSR REVERSE 20 55.17 40 5 3 TCGAGGAAATCATCTCAATC
CM001031.1 1 SSR FORWARD 20 55.14 55 1 0 CTCTCTCCCCACTCTCTCTT 238 3 0 CT 2 CTCTCTCTCTCTCT 14
CM001031.1 1 SSR REVERSE 20 55.17 40 5 3 TCGAGGAAATCATCTCAATC
CM001031.1 1 SSR FORWARD 20 55.14 55 1 0 CTCTCTCCCCACTCTCTCTT 238 3 0 CT 2 CTCTCTCTCTCTCT 14
CM001031.1 1 SSR REVERSE 20 55.17 40 5 3 TCGAGGAAATCATCTCAATC
CM001031.1 1 SSR FORWARD 20 55.14 55 1 0 CTCTCTCCCCACTCTCTCTT 238 3 0 CT 2 CTCTCTCTCTCTCT 14
CM001031.1 1 SSR REVERSE 20 55.17 40 5 3 TCGAGGAAATCATCTCAATC
CM001031.1 1 SSR FORWARD 20 55.14 55 1 0 CTCTCTCCCCACTCTCTCTT 238 3 0 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 42
CM001031.1 1 SSR REVERSE 20 55.17 40 5 3 TCGAGGAAATCATCTCAATC
CM001031.1 1 SSR FORWARD 20 55.22 40 3 3 GATTACGGTTTCAGGTTCAA 259 4 0 TA 2 TATATATATATA 12
CM001031.1 1 SSR REVERSE 20 55.17 40 4 0 ATCGTGGTAAAGCTACGAAA
CM001031.1 1 SSR FORWARD 20 55.05 45 2 0 CATCTTATCCTTCTGCCTTG 259 6 2 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 40
CM001031.1 1 SSR REVERSE 20 55.53 45 6 2 GGGTCGGTTATAAGGTGAAT
CM001031.1 1 SSR FORWARD 20 55.07 40 3 3 TCTGGGCTTGTTCCTAATAA 264 4 2 AT 2 ATATATATATATAT 14
CM001031.1 1 SSR REVERSE 20 55.63 45 3 0 TAGGCTTCACTTCTTTGGTG
CM001031.1 1 SSR FORWARD 20 55.87 45 3 3 TGAGAGAAGACTGGGTTCAA 245 5 2 GA 2 GAGAGAGAGAGA 12
CM001031.1 1 SSR REVERSE 20 55.11 50 4 1 AGAGATCAGAAACCCTAGCC
CM001031.1 1 SSR FORWARD 20 55.87 45 3 3 TGAGAGAAGACTGGGTTCAA 245 5 2 GA 2 GAGAGAGAGAGA 12
CM001031.1 1 SSR REVERSE 20 55.11 50 4 1 AGAGATCAGAAACCCTAGCC
CM001031.1 1 SSR FORWARD 20 55.87 45 3 3 TGAGAGAAGACTGGGTTCAA 245 5 2 GA 2 GAGAGAGAGAGAGA 14
CM001031.1 1 SSR REVERSE 20 55.11 50 4 1 AGAGATCAGAAACCCTAGCC
CM001031.1 1 SSR FORWARD 19 55.1 52.63 3 0 ATACTCTCACGCTGCTCCT 257 7 1 CT 2 CTCTCTCTCTCT 12
CM001031.1 1 SSR REVERSE 20 55.58 40 5 2 GAACTGCCTGAAAGTTGAAA
CM001031.1 1 SSR FORWARD 20 55.65 55 2 0 CTCCTCTCTTCTCGCTCTCT 244 4 1 CT 2 CTCTCTCTCTCTCTCT 16
CM001031.1 1 SSR REVERSE 20 55.16 40 5 3 AGAACTGCCTGAAAGTTGAA
CM001031.1 1 SSR FORWARD 20 55.65 55 2 0 CTCCTCTCTTCTCGCTCTCT 244 4 1 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 36
CM001031.1 1 SSR REVERSE 20 55.16 40 5 3 AGAACTGCCTGAAAGTTGAA
CM001031.1 1 SSR FORWARD 20 55.65 55 2 0 CTCCTCTCTTCTCGCTCTCT 244 4 1 CT 2 CTCTCTCTCTCTCTCTCT 18
CM001031.1 1 SSR REVERSE 20 55.16 40 5 3 AGAACTGCCTGAAAGTTGAA
CM001031.1 1 SSR FORWARD 20 55.65 55 2 0 CTCCTCTCTTCTCGCTCTCT 244 4 1 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 52
CM001031.1 1 SSR REVERSE 20 55.16 40 5 3 AGAACTGCCTGAAAGTTGAA
CM001031.1 1 SSR FORWARD 20 55.04 45 6 2 GAGCAATATTTCCTGACTCG 248 4 2 TA 2 TATATATATATA 12
CM001031.1 1 SSR REVERSE 20 55.75 45 3 2 GAGCCATCATCAACATTAGC
CM001031.1 1 SSR FORWARD 20 55.92 40 6 2 ATGAATCTTCCCATGGAACT 260 3 2 AG 2 AGAGAGAGAGAG 12
CM001031.1 1 SSR REVERSE 20 55.14 50 4 0 ATACACACGTCTCTCCCAAG
CM001031.1 1 SSR FORWARD 20 55.55 40 5 2 CCCATTGTGGATGTCATATT 251 4 2 TA 2 TATATATATATATATATA 18
CM001031.1 1 SSR REVERSE 20 55.01 50 3 0 GAGTATGGACCCTGTGAGAA
CM001031.1 1 SSR FORWARD 20 55.03 45 3 2 CCTTGCCCATAACACTAAAG 240 5 0 TC 2 TCTCTCTCTCTCTCTCTCTC 20
CM001031.1 1 SSR REVERSE 20 55.12 45 5 2 GTTATCAGATGGAACGGAGA
CM001031.1 1 SSR FORWARD 20 55.22 40 4 2 CAGGCTTGAGATTAAAATGC 259 3 1 AC 2 ACACACACACAC 12
CM001031.1 1 SSR REVERSE 20 55.09 50 3 2 CAAACGGGAGACTACTAACG
CM001031.1 1 SSR FORWARD 20 55.19 40 6 2 TAGCATGCACATCGTGTTAT 299 5 0 AT 2 ATATATATATAT 12
CM001031.1 1 SSR REVERSE 20 55.4 45 6 2 GCTATCATGAGCTTGTGGTT
CM001031.1 1 SSR FORWARD 20 55.56 40 4 0 CACGATCACTCAAACACAAA 238 3 0 CT 2 CTCTCTCTCTCTCT 14
CM001031.1 1 SSR REVERSE 20 55.03 45 2 0 TCATCTTCACCAACCCTAAC
CM001031.1 1 SSR FORWARD 20 55.58 45 1 0 AGGGAGTGGAAGAAGAAGAA 232 2 0 AG 2 AGAGAGAGAGAGAGAG 16
CM001031.1 1 SSR REVERSE 20 55.67 40 4 3 CCGAAAATGAAATACGTGAC
CM001031.1 1 SSR FORWARD 20 55.58 45 1 0 AGGGAGTGGAAGAAGAAGAA 232 2 0 GA 2 GAGAGAGAGAGA 12
CM001031.1 1 SSR REVERSE 20 55.67 40 4 3 CCGAAAATGAAATACGTGAC
CM001031.1 1 SSR FORWARD 20 55.16 40 3 2 TCTTGCTTCTTCAAACAGGT 256 4 0 AT 2 ATATATATATAT 12
CM001031.1 1 SSR REVERSE 20 55.49 45 2 0 GATGTTATGTTGCTGGGTTC
CM001031.1 1 SSR FORWARD 20 55.09 40 5 3 GTTGGCTTTTCAGATTCTTG 236 6 2 GA 2 GAGAGAGAGAGA 12
CM001031.1 1 SSR REVERSE 20 55.81 40 4 0 AAAATATCTCCAGCCAAACC
CM001031.1 1 SSR FORWARD 20 55.99 45 6 0 ATATACCGGTCTCGCTTTTC 247 4 2 TA 2 TATATATATATATATATATATATATA 26
CM001031.1 1 SSR REVERSE 20 55.12 45 2 0 ACGCAGACACAACATAGACA
CM001031.1 1 SSR FORWARD 20 55.49 45 3 1 TGCTAAACGGTAGTGAAACC 275 6 2 AT 2 ATATATATATATATAT 16
CM001031.1 1 SSR REVERSE 20 55.25 40 5 1 ACTCCCGTCAATTATTTCCT

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