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Chromosome3 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001028.1 1 SSR FORWARD 20 55.3 45 3 0 TGGTTTGACTAAAGGTCTGG 245 4 2 TC 2 TCTCTCTCTCTC 12
CM001028.1 1 SSR REVERSE 20 55.51 50 4 0 AGGTAGAACATACCGCTCCT
CM001028.1 1 SSR FORWARD 20 55.35 50 5 0 GAAAGGCTTACCTCGTCTCT 257 5 2 TG 2 TGTGTGTGTGTG 12
CM001028.1 1 SSR REVERSE 20 55.3 45 3 2 TGAATCCTCTCGGACAATAG
CM001028.1 1 SSR FORWARD 21 55.3 42.86 5 3 CTTTACGTTCAACAGCAAGAC 232 4 0 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 34
CM001028.1 1 SSR REVERSE 20 55.05 50 4 2 CTCTCTCAAGAACCAGATGC
CM001028.1 1 SSR FORWARD 20 55.22 40 4 2 AAGGCCCATAATAACTCCAT 249 4 3 CT 2 CTCTCTCTCTCTCTCTCT 18
CM001028.1 1 SSR REVERSE 20 55.21 50 2 2 GAGAGCGTGGTGATAGTGAT
CM001028.1 1 SSR FORWARD 20 55.38 40 6 0 ATCTTCACCTCATGATCCAA 249 5 0 AG 2 AGAGAGAGAGAG 12
CM001028.1 1 SSR REVERSE 20 55.15 45 4 1 TCACCACACCACATGATAAG
CM001028.1 1 SSR FORWARD 20 55.31 40 4 1 CGCTTACAATTTTCTCCAAG 241 7 2 TC 2 TCTCTCTCTCTCTCTCTCTC 20
CM001028.1 1 SSR REVERSE 20 55.09 40 8 0 GATCAAAAGCTTAAGCGAGA
CM001028.1 1 SSR FORWARD 20 55.44 40 3 1 AACTGAAAAACACGCTCATC 246 5 2 AT 2 ATATATATATATATATATATATATATAT 28
CM001028.1 1 SSR REVERSE 20 56.49 40 3 3 GCAACCAAAAATACTGAGCA
CM001028.1 1 SSR FORWARD 20 55.31 40 4 1 TTTTCCACCTTGGTTTACAG 208 3 1 AT 2 ATATATATATATATATAT 18
CM001028.1 1 SSR REVERSE 20 55.57 50 3 1 AACTTCTACACGGCTCACAG
CM001028.1 1 SSR FORWARD 20 55.12 40 5 1 AGAACTTCCGCAGTATGAAA 250 8 3 AT 2 ATATATATATATATATATATATATATAT 28
CM001028.1 1 SSR REVERSE 21 55.55 42.86 5 2 AAGTCTTCTACTGGGAACCAA
CM001028.1 1 SSR FORWARD 21 55.2 42.86 3 2 CCAAGAGCCATTTACTCCTAT 251 6 1 TA 2 TATATATATATATATA 16
CM001028.1 1 SSR REVERSE 20 55.7 40 6 0 TCGCGAAGAGTAATTCAAAC
CM001028.1 1 SSR FORWARD 20 55.16 40 3 2 GTTTGGCACAGTTGGTAAAT 277 4 2 TA 2 TATATATATATA 12
CM001028.1 1 SSR REVERSE 20 55.45 40 3 2 AAAGTTGCCTTGTTCATCTG
CM001028.1 1 SSR FORWARD 20 55.16 40 3 2 GTTTGGCACAGTTGGTAAAT 277 4 2 TA 2 TATATATATATA 12
CM001028.1 1 SSR REVERSE 20 55.45 40 3 2 AAAGTTGCCTTGTTCATCTG
CM001028.1 1 SSR FORWARD 20 55.61 45 4 2 AATTGGTAGCACGATAGCAG 238 5 0 AT 2 ATATATATATAT 12
CM001028.1 1 SSR REVERSE 19 55.06 42.11 2 0 GTGTGCTTATTGGGGTTTT
CM001028.1 1 SSR FORWARD 20 55.87 45 3 1 TGGAGGAGAAACAAGAGTCA 248 4 1 AG 2 AGAGAGAGAGAGAGAGAGAGAG 22
CM001028.1 1 SSR REVERSE 20 55.51 50 3 2 TAGACCCCATACAGTCCAGA
CM001028.1 1 SSR FORWARD 21 55.14 42.86 6 2 ACTAGTATCGTGCGGTTTACA 250 5 1 GA 2 GAGAGAGAGAGAGA 14
CM001028.1 1 SSR REVERSE 20 55.18 45 4 1 GGCATGATCTTCAACACTCT
CM001028.1 1 SSR FORWARD 19 56.01 42.11 4 2 CCCAAACCTAATCGAATCA 280 5 2 AT 2 ATATATATATATATATATAT 20
CM001028.1 1 SSR REVERSE 19 55.15 42.11 4 2 GGAGTAAACATGCCGATTT
CM001028.1 1 SSR FORWARD 20 55.39 40 5 2 TTGCTTAGGAATGGAGCTTA 250 4 2 CA 2 CACACACACACACA 14
CM001028.1 1 SSR REVERSE 20 55.12 40 4 2 TGGTCTTTGACGAGCTTTAT
CM001028.1 1 SSR FORWARD 20 55.54 40 6 2 CAACGAGTTCAACGTTTTCT 234 8 2 TA 2 TATATATATATA 12
CM001028.1 1 SSR REVERSE 20 55.2 40 4 2 AACTCGTTCATTTGGATCAG
CM001028.1 1 SSR FORWARD 19 55.15 47.37 2 0 TAGGGAGAAGGAAGAGCAA 270 5 1 GA 2 GAGAGAGAGAGAGAGAGAGAGA 22
CM001028.1 1 SSR REVERSE 20 56.53 45 3 0 TACACCTCGTCGCTAAAATG
CM001028.1 1 SSR FORWARD 18 55.29 50 4 1 GTTCTCATATCGCCCACA 247 4 0 AC 2 ACACACACACACACACAC 18
CM001028.1 1 SSR REVERSE 20 55.81 40 4 1 AATTGGATTAGTTGGCTTCC
CM001028.1 1 SSR FORWARD 19 55.58 57.89 1 0 CACACACACCACACTCTCC 257 2 0 TC 2 TCTCTCTCTCTCTCTCTC 18
CM001028.1 1 SSR REVERSE 20 55.26 40 4 0 CTCCTTCTTCCTTTCGATTT
CM001028.1 1 SSR FORWARD 20 55.96 40 2 2 GTTTATTGGCTTTCCCCTTA 245 4 2 AT 2 ATATATATATATATAT 16
CM001028.1 1 SSR REVERSE 20 56.26 40 3 2 CCTTCCATCAGCATTGTAAA
CM001028.1 1 SSR FORWARD 20 55.23 45 6 3 GTCACTTATGCGACTCCATT 240 4 2 TA 2 TATATATATATA 12
CM001028.1 1 SSR REVERSE 20 55.12 40 4 2 ATTCTCTAAAGCAACGTGGA
CM001028.1 1 SSR FORWARD 20 55.12 40 3 2 ACCAACACAGAAAATCCTTG 253 6 2 AT 2 ATATATATATATAT 14
CM001028.1 1 SSR REVERSE 20 55.38 40 2 1 GGCAGTTGTCCTTATTTTCA
CM001028.1 1 SSR FORWARD 19 55.33 47.37 4 3 CCAAGAGAACTCCAAAGGA 254 5 1 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 46
CM001028.1 1 SSR REVERSE 20 55.26 45 7 2 CCTCATGGGCTATGAAGATA
CM001028.1 1 SSR FORWARD 20 56.13 45 3 2 GGCAGCATAAGGAAGAAGAT 248 5 1 AT 2 ATATATATATATATATAT 18
CM001028.1 1 SSR REVERSE 20 55.03 45 4 0 ATGTCGCACCTATATGAACC
CM001028.1 1 SSR FORWARD 20 55.37 45 8 2 GGACGAAATCTCGTTCACTA 250 4 3 AG 2 AGAGAGAGAGAGAGAG 16
CM001028.1 1 SSR REVERSE 20 55.28 45 4 1 AAGAGCAGAGCTTGGTATGA
CM001028.1 1 SSR FORWARD 20 55.39 50 4 2 ACAACTCTCCCTAACCGAGT 234 5 1 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 54
CM001028.1 1 SSR REVERSE 20 55.31 45 6 2 GAGTGTAACTCGCACCATTT
CM001028.1 1 SSR FORWARD 20 55.8 40 4 3 TGAAACTCTGAACCACGTTT 270 4 3 TA 2 TATATATATATA 12
CM001028.1 1 SSR REVERSE 20 55.05 40 4 1 GGACTTTGGGACAATACAAA
CM001028.1 1 SSR FORWARD 20 55.87 50 5 2 GAGTCTCTTTGACCGGATCT 248 4 1 GA 2 GAGAGAGAGAGAGAGAGAGA 20
CM001028.1 1 SSR REVERSE 20 55.2 40 4 2 TTCACACCAATGAACAGCTA
CM001028.1 1 SSR FORWARD 20 55.14 40 3 1 GGCTTTCCAACTTTCCTATT 245 3 0 CT 2 CTCTCTCTCTCT 12
CM001028.1 1 SSR REVERSE 20 55.67 50 4 0 CATGGTCTATCACTGCCTTC
CM001028.1 1 SSR FORWARD 20 55.35 45 7 3 TTATAGCTGGTCCATCTTGG 264 5 2 AT 2 ATATATATATAT 12
CM001028.1 1 SSR REVERSE 20 55.47 40 5 1 CACCAATGATTTGAAAGGTC
CM001028.1 1 SSR FORWARD 20 55.2 40 8 2 TGATGTTAACTTCGTCGTGA 238 5 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 52
CM001028.1 1 SSR REVERSE 20 55.38 40 2 0 GGCAAAACTATGAAGAACCA
CM001028.1 1 SSR FORWARD 20 55.15 45 2 0 TCTTCTCCACCATCAGTTTC 202 6 2 TC 2 TCTCTCTCTCTC 12
CM001028.1 1 SSR REVERSE 21 55.71 42.86 6 3 GGAGTCAACATCTTGGAGATT
CM001028.1 1 SSR FORWARD 21 55.04 42.86 5 1 CAACGGTTTAGGTGTAAGTTG 250 5 3 GA 2 GAGAGAGAGAGAGAGAGAGA 20
CM001028.1 1 SSR REVERSE 20 55.67 40 4 0 AATCAACTCGGATACGTCAA
CM001028.1 1 SSR FORWARD 20 55.25 40 2 2 ATAAGCAAGCGAAGACCATA 260 3 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 58
CM001028.1 1 SSR REVERSE 19 55.36 52.63 3 0 GAGAGACCTGGGATGAATG
CM001028.1 1 SSR FORWARD 20 55.22 45 4 2 CACAAAAGCTAGAACCCTTG 241 4 0 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 58
CM001028.1 1 SSR REVERSE 20 55.74 55 4 1 CTGTGTGATCTCTCCCTCTG
CM001028.1 1 SSR FORWARD 20 55.38 45 2 2 TCTCAACTATCCCTTTCACG 238 4 1 AT 2 ATATATATATAT 12
CM001028.1 1 SSR REVERSE 22 56.03 40.91 5 1 CAGGAAACAATGACATATACCC
CM001028.1 1 SSR FORWARD 20 55.03 40 6 0 TAACAACGCTGTGACTTTTG 258 5 0 AT 2 ATATATATATATATATAT 18
CM001028.1 1 SSR REVERSE 20 55.02 50 6 2 CCGGTGTACATGTTAGGACT
CM001028.1 1 SSR FORWARD 20 55.5 45 5 3 GACGCATGACATTGACTGTA 250 4 1 AT 2 ATATATATATATAT 14
CM001028.1 1 SSR REVERSE 22 55.44 40.91 4 1 TGTACCCTAACATGGAGTTCTT
CM001028.1 1 SSR FORWARD 20 55.5 45 5 3 GACGCATGACATTGACTGTA 250 4 1 AT 2 ATATATATATAT 12
CM001028.1 1 SSR REVERSE 22 55.44 40.91 4 1 TGTACCCTAACATGGAGTTCTT
CM001028.1 1 SSR FORWARD 20 55.81 45 4 2 GGTTTCGGTCCAAGTAGTTT 256 4 1 AT 2 ATATATATATATATATAT 18
CM001028.1 1 SSR REVERSE 20 55.77 45 7 3 CATAGCTGCAGTGGCTATTT
CM001028.1 1 SSR FORWARD 20 55.1 45 2 0 CATCTTCTGTGGAGTTTGGT 250 3 2 AG 2 AGAGAGAGAGAGAG 14
CM001028.1 1 SSR REVERSE 20 55.31 50 4 1 CTTCTTCTTCGCTGAGACAC
CM001028.1 1 SSR FORWARD 20 55.81 40 4 2 AAATTCTGAGTCCATGAGCA 227 3 2 TGG 3 TGGTGGTGGTGG 12
CM001028.1 1 SSR REVERSE 20 55.28 40 4 1 GCACCAAATCTTTCACTGTT
CM001028.1 1 SSR FORWARD 20 55.94 40 4 2 GATTCCTTCTCGTTTTCGAT 235 7 3 AAT 3 AATAATAATAAT 12
CM001028.1 1 SSR REVERSE 20 55.41 50 5 0 GAGAAGGGCCTACCAAATAC
CM001028.1 1 SSR FORWARD 20 55.38 40 7 3 TGTTTCTGTCACCAACAATG 240 4 1 ATT 3 ATTATTATTATT 12
CM001028.1 1 SSR REVERSE 20 55.14 50 4 2 AGTTCAGTCCAGTGATACGG
CM001028.1 1 SSR FORWARD 20 55.12 45 5 2 CTCTCAACGGCTGAATTAAC 256 4 0 AAC 3 AACAACAACAACAAC 15
CM001028.1 1 SSR REVERSE 20 55.04 45 5 0 TGTGACAGGTGAGTGAAAGA
CM001028.1 1 SSR FORWARD 20 55.1 45 4 3 TCTCAGTGCAAACTGCTATG 261 3 1 TAA 3 TAATAATAATAATAA 15
CM001028.1 1 SSR REVERSE 20 55.1 50 2 0 TAACACACACCCTCTTCTCC
CM001028.1 1 SSR FORWARD 20 55.61 45 4 1 ACAGCGGTATGTGAGAATGT 255 4 1 CTT 3 CTTCTTCTTCTT 12
CM001028.1 1 SSR REVERSE 20 55.25 40 2 2 ACCATAGACGAAAAATCACG
CM001028.1 1 SSR FORWARD 20 55.13 45 6 3 CAGAGGGTGGATATCTTCAA 247 5 2 TTTG 4 TTTGTTTGTTTG 12
CM001028.1 1 SSR REVERSE 20 55.11 45 6 2 AACTGTAAGCTTGCGAAGAG
CM001028.1 1 SSR FORWARD 20 55.03 40 6 0 TAACAACGCTGTGACTTTTG 258 5 0 ATACAT 6 ATACATATACATATACAT 18
CM001028.1 1 SSR REVERSE 20 55.02 50 6 2 CCGGTGTACATGTTAGGACT
CM001028.1 1 SSR FORWARD 20 55.77 45 7 1 ATCACATGATGTTCCTCGTC 267 4 1 TC 2 TCTCTCTCTCTCTCTC 16
CM001028.1 1 SSR REVERSE 20 55.02 45 4 2 ATCTGAGCCGTTGATCTCTA
CM001028.1 1 SSR FORWARD 20 55.81 40 4 2 TCCGTCAATTCCTCTGTTTA 286 5 1 AT 2 ATATATATATATATATAT 18
CM001028.1 1 SSR REVERSE 20 55.24 40 4 2 GTAAATCTGTCGGTGCAAAT
CM001028.1 1 SSR FORWARD 20 55.09 45 2 0 CTTCATCTTCCTCCTCCTTT 246 4 0 TC 2 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC 32
CM001028.1 1 SSR REVERSE 20 55.19 40 2 2 GTGATTTGTTTGCTGGAGAT
CM001028.1 1 SSR FORWARD 20 55.43 40 3 0 TTCCAAAACTTCTTCTGTGG 270 3 0 TA 2 TATATATATATATATA 16
CM001028.1 1 SSR REVERSE 20 55.43 40 3 0 TTCCAAAACTTCTTCTGTGG
CM001028.1 1 SSR FORWARD 20 56.08 40 6 0 AACCAATTTGTACCCGACTT 280 7 1 AT 2 ATATATATATATATATATATAT 22
CM001028.1 1 SSR REVERSE 19 55.44 47.37 2 2 GGGTTCGGGTATTTAGGAT
CM001028.1 1 SSR FORWARD 21 56.28 42.86 7 2 GCAAAAGACTCATTAGCCACT 297 5 1 TA 2 TATATATATATATA 14
CM001028.1 1 SSR REVERSE 20 55.05 40 2 1 GGTAGGTTTTTGGAATGTGA
CM001028.1 1 SSR FORWARD 20 55.07 45 4 0 AATGGTGAGAATACGTGGAG 251 5 2 AT 2 ATATATATATATATATATAT 20
CM001028.1 1 SSR REVERSE 20 55.7 50 2 2 GCCCAATACTCATACACTCG
CM001028.1 1 SSR FORWARD 23 55.03 43.48 5 2 GGTACAAACTCTCTTTCTACGTG 240 5 1 TA 2 TATATATATATATATA 16
CM001028.1 1 SSR REVERSE 20 56.39 40 2 0 TGTGTGCTCTTTTTGTTTCC
CM001028.1 1 SSR FORWARD 20 55.21 50 2 0 CCGTAGTTCCCTCTTTCTCT 266 3 0 AT 2 ATATATATATATATAT 16
CM001028.1 1 SSR REVERSE 20 56.63 50 3 2 TCTCAGTCGTCACTTTCTCG
CM001028.1 1 SSR FORWARD 20 55.32 45 4 1 ATTATGAGCATCGTGAGAGG 256 5 0 AT 2 ATATATATATATATATATAT 20
CM001028.1 1 SSR REVERSE 20 55.09 40 4 1 ATGCCCTTTATACTCAGCAA
CM001028.1 1 SSR FORWARD 20 55.32 45 4 1 ATTATGAGCATCGTGAGAGG 256 5 0 TG 2 TGTGTGTGTGTGTGTGTGTGTG 22
CM001028.1 1 SSR REVERSE 20 55.09 40 4 1 ATGCCCTTTATACTCAGCAA
CM001028.1 1 SSR FORWARD 20 55.05 45 4 2 TTGTCAGCTTGGAGAGAGAT 253 6 3 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 34
CM001028.1 1 SSR REVERSE 20 55.43 40 3 0 CTTCCTTCACCAAGACAAAA
CM001028.1 1 SSR FORWARD 21 55.18 42.86 7 3 GATAGGCTATTGCAAACACTG 250 4 0 AT 2 ATATATATATAT 12
CM001028.1 1 SSR REVERSE 20 56.07 40 3 1 ATTATCCTCCACCTTCCAAA
CM001028.1 1 SSR FORWARD 20 55.43 40 4 0 TAATGGTTTTAGCCACCAAG 249 5 0 TA 2 TATATATATATATATATA 18
CM001028.1 1 SSR REVERSE 20 55.3 45 6 2 GTTTGGTATACATGGGATCG
CM001028.1 1 SSR FORWARD 20 55.43 40 4 0 TAATGGTTTTAGCCACCAAG 249 5 0 TA 2 TATATATATATATATATA 18
CM001028.1 1 SSR REVERSE 20 55.3 45 6 2 GTTTGGTATACATGGGATCG
CM001028.1 1 SSR FORWARD 20 55.33 45 4 2 TTGTTCTTCCTCTCATCGAC 251 3 1 AT 2 ATATATATATAT 12
CM001028.1 1 SSR REVERSE 20 55.38 40 3 3 GCCTGTATGTTTTCCTTGAA
CM001028.1 1 SSR FORWARD 20 55.17 40 5 1 AATAATTCAGTGGCATGAGG 218 4 1 AT 2 ATATATATATATAT 14
CM001028.1 1 SSR REVERSE 20 55.42 40 4 1 TGTTTGTTGGAATACCCAGT
CM001028.1 1 SSR FORWARD 20 55 40 6 0 CCTTAAATTTTGGAGGAGGT 236 6 3 TA 2 TATATATATATATATATA 18
CM001028.1 1 SSR REVERSE 20 56.44 45 4 2 ATGAAAATCTACCCCACGTC

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