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Chromosome12 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001037.1 1 SSR REVERSE 20 55.94 40 6 2 AACGCACATGAGCTTGTAAT
CM001037.1 1 SSR FORWARD 20 55.3 40 8 2 CTTGCAAGATTTTACCAAGC 248 3 1 AG 2 AGAGAGAGAGAG 12
CM001037.1 1 SSR REVERSE 20 55.31 50 6 2 CCGACAGTCTCGAGTTGTAT
CM001037.1 1 SSR FORWARD 20 55.86 40 3 0 AAATCCGAAAGAGGAAACAC 254 5 3 AG 2 AGAGAGAGAGAGAGAGAGAG 20
CM001037.1 1 SSR REVERSE 21 55.08 42.86 3 0 CACAGTTTCACTTATGGTGGT
CM001037.1 1 SSR FORWARD 20 56.21 45 4 2 CTAAAACGTATCACGGTCCA 252 5 2 AG 2 AGAGAGAGAGAG 12
CM001037.1 1 SSR REVERSE 23 55.26 43.48 6 3 CTCAGGCTTGTAAGACTTGATAG
CM001037.1 1 SSR FORWARD 20 55.18 45 4 2 AACATCAAGGAGATCACTGC 256 3 2 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 20 55.12 45 2 2 CCGAACATAAGAACAGAAGC
CM001037.1 1 SSR FORWARD 20 55.03 40 4 2 GATCCGCGATGTAATTTTAG 265 4 2 GT 2 GTGTGTGTGTGT 12
CM001037.1 1 SSR REVERSE 20 55.9 45 4 2 CCCCCTAGAAAAACCACTTA
CM001037.1 1 SSR FORWARD 20 55.16 40 2 1 TTGCTCTTCTTGTTTCCAGT 247 5 3 TG 2 TGTGTGTGTGTGTG 14
CM001037.1 1 SSR REVERSE 20 55.87 40 4 0 TCGAGAATGAAGATTGAGGA
CM001037.1 1 SSR FORWARD 20 55.25 40 8 3 AAATGGCATTCCAGTTACAG 280 4 1 AT 2 ATATATATATATAT 14
CM001037.1 1 SSR REVERSE 21 55.16 42.86 4 0 AATGGTAGCAAGACATCACAC
CM001037.1 1 SSR FORWARD 20 55.6 40 6 2 CATGCATAATCACAGTCGAA 254 3 0 AT 2 ATATATATATAT 12
CM001037.1 1 SSR REVERSE 20 55.35 45 4 2 CATCTAGCCTGAACCCAATA
CM001037.1 1 SSR FORWARD 20 55.25 40 8 3 AAATGGCATTCCAGTTACAG 280 4 1 AT 2 ATATATATATATAT 14
CM001037.1 1 SSR REVERSE 21 55.16 42.86 4 0 AATGGTAGCAAGACATCACAC
CM001037.1 1 SSR FORWARD 20 55.6 40 6 2 CATGCATAATCACAGTCGAA 254 3 0 AT 2 ATATATATATAT 12
CM001037.1 1 SSR REVERSE 20 55.35 45 4 2 CATCTAGCCTGAACCCAATA
CM001037.1 1 SSR FORWARD 20 55.09 45 6 0 CATGCATAGCTTTACCTTCC 251 5 0 GA 2 GAGAGAGAGAGA 12
CM001037.1 1 SSR REVERSE 20 55.82 45 4 2 CTCCCCGTCTCAATCTATTT
CM001037.1 1 SSR FORWARD 20 56.01 45 8 2 CGTCGACGAAATAGATTGAG 233 3 2 GA 2 GAGAGAGAGAGA 12
CM001037.1 1 SSR REVERSE 20 56.31 40 3 2 TAAAGAGAATGCGGTGCTTA
CM001037.1 1 SSR FORWARD 20 56.01 45 8 2 CGTCGACGAAATAGATTGAG 233 3 2 GA 2 GAGAGAGAGAGA 12
CM001037.1 1 SSR REVERSE 20 56.31 40 3 2 TAAAGAGAATGCGGTGCTTA
CM001037.1 1 SSR FORWARD 20 55.67 45 6 0 GCCCATATGTTCTGTTCTTG 246 5 1 TA 2 TATATATATATATA 14
CM001037.1 1 SSR REVERSE 20 55.08 40 7 3 AACCAACATTGCTTGGATAC
CM001037.1 1 SSR FORWARD 20 55.28 40 5 3 ATGACATTACATGGCCTCAT 219 5 3 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 20 55.87 45 4 2 TGGGATCTTGGATGTCTATG
CM001037.1 1 SSR FORWARD 20 55.33 40 5 2 CAGGTTTGGCACAACTAAAT 234 4 1 AT 2 ATATATATATAT 12
CM001037.1 1 SSR REVERSE 22 55.71 40.91 4 0 AGTTACATGGGTGTCTAAATGG
CM001037.1 1 SSR FORWARD 21 55.22 42.86 4 3 TCACAGGACTCAATACGAATC 242 4 0 TC 2 TCTCTCTCTCTCTC 14
CM001037.1 1 SSR REVERSE 20 55.95 40 7 2 ACAAACTTTGGGCCTTAAAC
CM001037.1 1 SSR FORWARD 20 55.28 40 5 3 ATGACATTACATGGCCTCAT 219 5 3 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 20 55.87 45 4 2 TGGGATCTTGGATGTCTATG
CM001037.1 1 SSR FORWARD 20 55.33 40 5 2 CAGGTTTGGCACAACTAAAT 234 4 1 AT 2 ATATATATATAT 12
CM001037.1 1 SSR REVERSE 22 55.71 40.91 4 0 AGTTACATGGGTGTCTAAATGG
CM001037.1 1 SSR FORWARD 21 55.22 42.86 4 3 TCACAGGACTCAATACGAATC 242 4 0 TC 2 TCTCTCTCTCTCTC 14
CM001037.1 1 SSR REVERSE 20 55.95 40 7 2 ACAAACTTTGGGCCTTAAAC
CM001037.1 1 SSR FORWARD 20 55.38 40 3 2 ATCGCATCTTTTACCATACG 265 6 2 AG 2 AGAGAGAGAGAG 12
CM001037.1 1 SSR REVERSE 20 55.11 45 5 2 AGAGATGCTGACCGTCTTTA
CM001037.1 1 SSR FORWARD 20 55.44 40 3 2 GCAGAACAACGAACTGAAAT 246 3 3 AC 2 ACACACACACACAC 14
CM001037.1 1 SSR REVERSE 19 55.05 42.11 3 1 AGAAGCAGCCATTACGATT
CM001037.1 1 SSR FORWARD 20 55.36 45 3 2 GCGGAAGTTAGAGAAAGGTT 256 6 3 AG 2 AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG 52
CM001037.1 1 SSR REVERSE 20 55.2 50 0 0 TTCTCTCCTCTCTCCTTTCC
CM001037.1 1 SSR FORWARD 20 55.23 50 4 2 ACTCTATCCCGAGGAGACAT 253 3 1 CT 2 CTCTCTCTCTCTCTCT 16
CM001037.1 1 SSR REVERSE 20 55.42 45 7 3 CCATAACCAACTGTGGTTTC
CM001037.1 1 SSR FORWARD 20 55.74 45 5 2 TCTATCCCGAAGAGACATGA 252 3 2 CT 2 CTCTCTCTCTCTCTCT 16
CM001037.1 1 SSR REVERSE 20 55.42 45 7 3 CCATAACCAACTGTGGTTTC
CM001037.1 1 SSR FORWARD 20 55.77 45 6 2 TCTGTAGATGCACAACAACG 263 5 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 46
CM001037.1 1 SSR REVERSE 20 55.67 45 4 2 TGGTGCTCCTTGTCATATCT
CM001037.1 1 SSR FORWARD 20 55.14 55 3 0 CGGAGAACAGTGAGAGAGAG 233 3 0 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 30
CM001037.1 1 SSR REVERSE 20 55.18 40 4 2 TCTTGAGGAGATTGGCTAAA
CM001037.1 1 SSR FORWARD 20 55.45 45 4 2 AAGAAGTCCATCAAGTGCAG 248 7 2 TG 2 TGTGTGTGTGTG 12
CM001037.1 1 SSR REVERSE 20 55.21 40 2 2 ACAAGAAGGGAGATGGATTT
CM001037.1 1 SSR FORWARD 20 55.2 55 2 2 ACACAGCACCAGTAGACCTC 249 4 2 AT 2 ATATATATATATATATATAT 20
CM001037.1 1 SSR REVERSE 20 55.3 40 4 0 GCTCCAGCTTTAATGTTTTG
CM001037.1 1 SSR FORWARD 21 55.07 42.86 6 0 AAAAGTGTCTCTAGGGATTGG 220 3 0 TC 2 TCTCTCTCTCTCTC 14
CM001037.1 1 SSR REVERSE 20 55.47 40 5 3 GTTGAGTGAAATGGGATTTG
CM001037.1 1 SSR FORWARD 20 56.06 50 6 1 CTCAGACCGGTACACCATAA 234 4 0 CT 2 CTCTCTCTCTCTCT 14
CM001037.1 1 SSR REVERSE 20 55.32 50 3 0 GAGAATCAGAGGACGAGACA
CM001037.1 1 SSR FORWARD 20 56.06 55 2 0 CGACATCCTCCTCTCTCTCT 238 4 0 TC 2 TCTCTCTCTCTC 12
CM001037.1 1 SSR REVERSE 20 55.22 45 3 0 GAACCTAATCAAAGGGGAAC
CM001037.1 1 SSR FORWARD 20 55.33 40 5 2 CCACGACGTTAATTTTGACT 257 4 0 GA 2 GAGAGAGAGAGAGA 14
CM001037.1 1 SSR REVERSE 20 55.05 50 6 2 GTCATAGCCTCCGCTATATG
CM001037.1 1 SSR FORWARD 20 55.33 40 5 2 CCACGACGTTAATTTTGACT 257 4 0 GA 2 GAGAGAGAGAGAGA 14
CM001037.1 1 SSR REVERSE 20 55.05 50 6 2 GTCATAGCCTCCGCTATATG
CM001037.1 1 SSR FORWARD 20 55.83 40 4 0 GGGGCACGTTATATTTTCTT 262 3 1 AT 2 ATATATATATAT 12
CM001037.1 1 SSR REVERSE 20 55.53 45 6 2 GCTAGCACCCGTATCAATTA
CM001037.1 1 SSR FORWARD 20 55.11 40 4 3 GACACGTCCACTTTCAATTT 244 4 2 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 21 55.93 42.86 4 2 AATCCACCAGCAAATACTAGG
CM001037.1 1 SSR FORWARD 20 55.84 45 5 3 CTCCTATGCTTCAACCAATG 256 5 3 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 20 55.8 40 8 0 GCATGCATATGTAAGCACAA
CM001037.1 1 SSR FORWARD 19 55.13 47.37 6 2 GGTCAAGACCATCGCTATT 294 4 1 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 20 55.09 40 3 2 ATGGAGGGGTAACAAAATCT
CM001037.1 1 SSR FORWARD 20 55.14 45 4 0 GCTAGTTGATTGGTGTTGGT 247 6 0 AC 2 ACACACACACAC 12
CM001037.1 1 SSR REVERSE 20 55.23 40 5 1 ATCAACCACTGGAAATGTGT
CM001037.1 1 SSR FORWARD 20 55.06 45 8 0 TGGATGAATTCTCTCCACTC 250 5 2 CT 2 CTCTCTCTCTCTCTCTCTCT 20
CM001037.1 1 SSR REVERSE 20 56.72 40 6 2 ACCTTCACCATCAATGGAAT
CM001037.1 1 SSR FORWARD 20 56.94 40 5 2 CTTTTGCCAACCTTTGAGAT 299 5 0 TA 2 TATATATATATATATA 16
CM001037.1 1 SSR REVERSE 20 55.97 40 4 1 TGCAAACTGGTCTTCTTTGT
CM001037.1 1 SSR FORWARD 21 55.66 47.62 2 0 GTCTAATCCTCACCACAACCT 264 4 2 GA 2 GAGAGAGAGAGAGAGAGA 18
CM001037.1 1 SSR REVERSE 20 55.65 45 5 0 AGCGATTCGTGTAAGTGAAG
CM001037.1 1 SSR FORWARD 20 55.84 45 5 3 CTCCTATGCTTCAACCAATG 256 5 3 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 20 55.8 40 8 0 GCATGCATATGTAAGCACAA
CM001037.1 1 SSR FORWARD 19 55.13 47.37 6 2 GGTCAAGACCATCGCTATT 294 4 1 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 20 55.09 40 3 2 ATGGAGGGGTAACAAAATCT
CM001037.1 1 SSR FORWARD 20 55.14 45 4 0 GCTAGTTGATTGGTGTTGGT 247 6 0 AC 2 ACACACACACAC 12
CM001037.1 1 SSR REVERSE 20 55.23 40 5 1 ATCAACCACTGGAAATGTGT
CM001037.1 1 SSR FORWARD 20 55.06 45 8 0 TGGATGAATTCTCTCCACTC 250 5 2 CT 2 CTCTCTCTCTCTCTCTCTCT 20
CM001037.1 1 SSR REVERSE 20 56.72 40 6 2 ACCTTCACCATCAATGGAAT
CM001037.1 1 SSR FORWARD 20 56.94 40 5 2 CTTTTGCCAACCTTTGAGAT 299 5 0 TA 2 TATATATATATATATA 16
CM001037.1 1 SSR REVERSE 20 55.97 40 4 1 TGCAAACTGGTCTTCTTTGT
CM001037.1 1 SSR FORWARD 21 55.66 47.62 2 0 GTCTAATCCTCACCACAACCT 264 4 2 GA 2 GAGAGAGAGAGAGAGAGA 18
CM001037.1 1 SSR REVERSE 20 55.65 45 5 0 AGCGATTCGTGTAAGTGAAG
CM001037.1 1 SSR FORWARD 20 55.6 40 5 3 CATCGCAACAACATCAGATA 239 4 3 TG 2 TGTGTGTGTGTG 12
CM001037.1 1 SSR REVERSE 20 55.12 40 4 0 TCCAGCTTTCTGTAATTCGT
CM001037.1 1 SSR FORWARD 20 55.31 40 4 1 CTTCTTATTCCATTCGACCA 218 5 1 TA 2 TATATATATATA 12
CM001037.1 1 SSR REVERSE 19 55.48 42.11 4 2 ATGAACAAGCCCAAGTCAT

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