Detection of Rotavirus in Diarrheic Bovine Calves by RNA-PAGE: Detection of rotavirus

SAVITA KUMARI; ANJAY .; MANOJ KUMAR; P KAUSHIK; SUDHA KUMARI; PANKAJ KUMAR

Authors

SAVITA KUMARI Bihar Veterinary College, Patna, (BASU,Patna)
ANJAY . Department of Veterinary Public Health and Epidemiology Bihar Veterinary College, BASU, Patna, Bihar
MANOJ KUMAR Department of Veterinary Microbiology, Bihar Veterinary College, Patna, (BASU,Patna)
P KAUSHIK Department of Veterinary Public Health and Epidemiology, Bihar Veterinary College, Patna, (BASU,Patna)
SUDHA KUMARI Department of Veterinary Microbiology, Bihar Veterinary College, Patna, (BASU,Patna)
PANKAJ KUMAR Department of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar

Abstract

Rotavirus is an important infectious agent causing neonatal diarrhea in bovine calves. The present study was conducted to know the prevalence of rotavirus circulating in dairy herds in Patna and adjacent areas of Bihar. A total of 96 diarrheic fecal samples were collected from cow calves (n=67) and buffalo calves (n=29) up to 6months of age group exhibiting diarrhea during the period from November 2014 to March 2015 and screened for the presence of rotavirus using RNA-PAGE. Nine samples were found positive having electrophoretic pattern of 4:2:3:2 on gel that corresponded to mammalian group A rotavirus. The overall prevalence of Group A rotavirus in diarrheic calves was found to be 09.37%. The results showed the presence of rotavirus in bovine calves of this part of the state and suggested for further elaborative studies to collect better epidemiological and molecular data about the circulating rotavirus strains.

Keywords: Bovine calves, Diarrhea, Group A rotavirus, Prevalence, RNA-PAGE.

Author Biographies

SAVITA KUMARI, Bihar Veterinary College, Patna, (BASU,Patna)

Bihar Veterinary College, Patna, (BASU,Patna)

ANJAY ., Department of Veterinary Public Health and Epidemiology Bihar Veterinary College, BASU, Patna, Bihar

Department of Veterinary Public Health and Epidemiology

Bihar Veterinary College, BASU, Patna, Bihar

MANOJ KUMAR, Department of Veterinary Microbiology, Bihar Veterinary College, Patna, (BASU,Patna)

Department of Veterinary Microbiology, Bihar Veterinary College, Patna, (BASU,Patna)

P KAUSHIK, Department of Veterinary Public Health and Epidemiology, Bihar Veterinary College, Patna, (BASU,Patna)

Department of Veterinary Public Health and Epidemiology, Bihar Veterinary College, Patna, (BASU,Patna)

SUDHA KUMARI, Department of Veterinary Microbiology, Bihar Veterinary College, Patna, (BASU,Patna)

Department of Veterinary Microbiology, Bihar Veterinary College, Patna, (BASU,Patna)

PANKAJ KUMAR, Department of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar

Department of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar

Detection of Rotavirus in Diarrheic Bovine Calves by RNA-PAGE