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Chromosome16 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001041.1 1 SSR FORWARD 20 55.52 45 6 2 AGGCCTTTAAGAAAGCTCAC 246 5 0 AT 2 ATATATATATAT 12
CM001041.1 1 SSR REVERSE 19 55.11 47.37 4 0 TCGGACCAGAACCTTAATC
CM001041.1 1 SSR FORWARD 20 55.13 45 6 1 ATTTACCCGGGAGATAGTGT 218 4 1 AT 2 ATATATATATAT 12
CM001041.1 1 SSR REVERSE 20 55.07 40 4 0 TCTCTTCACTTGCATCCTTT
CM001041.1 1 SSR FORWARD 20 55.32 45 3 2 CTGCTAACTTCACCCAACAT 232 6 2 CA 2 CACACACACACA 12
CM001041.1 1 SSR REVERSE 20 55.19 50 6 2 GGTGGAGAAAGCTTAGAGGT
CM001041.1 1 SSR FORWARD 20 55.7 45 6 2 GAATCCAAGACCTTGACCTT 236 5 1 AG 2 AGAGAGAGAGAGAGAG 16
CM001041.1 1 SSR REVERSE 20 55.05 40 4 0 TGTAATCAAGATGTGCGTGT
CM001041.1 1 SSR FORWARD 20 55.58 45 4 3 TGCTTCCATTTGCTAGTACC 249 3 1 GA 2 GAGAGAGAGAGA 12
CM001041.1 1 SSR REVERSE 20 55.92 45 4 3 CCGTGTTATTCTTCGACAAG
CM001041.1 1 SSR FORWARD 19 55.01 52.63 2 1 GGTGGTAGGTGTGTGTTGA 245 3 1 TG 2 TGTGTGTGTGTGTGTGTGTG 20
CM001041.1 1 SSR REVERSE 20 55.33 40 5 2 AAGTGCGGCATAAGAACTAA
CM001041.1 1 SSR FORWARD 20 55.49 45 4 1 GGCATGGAATGTAAGTTGTC 266 4 2 TA 2 TATATATATATATATATATATATATA 26
CM001041.1 1 SSR REVERSE 20 55.26 50 3 2 GGGGGTAAAGAGAACCATAG
CM001041.1 1 SSR FORWARD 20 55.06 40 6 2 AATATGGCCAACACCTTATG 251 5 2 TA 2 TATATATATATATATA 16
CM001041.1 1 SSR REVERSE 20 55.83 40 4 0 TAATCTCTGGTGTGCGATTT
CM001041.1 1 SSR FORWARD 18 56.79 44.44 8 2 ATCCGGATTTGGAACCTT 294 5 3 AT 2 ATATATATATAT 12
CM001041.1 1 SSR REVERSE 19 55.93 42.11 6 1 AATGTTGGATCCGTCAAAG
CM001041.1 1 SSR FORWARD 20 55.02 45 4 1 TCTCTCGGCTGATTGTATCT 252 3 1 TA 2 TATATATATATA 12
CM001041.1 1 SSR REVERSE 20 55.73 40 6 0 TAATGGATCTTCGAACAGGA
CM001041.1 1 SSR FORWARD 20 56.08 40 4 3 ATTCGGTCTGGAATGAGATT 252 5 2 TA 2 TATATATATATA 12
CM001041.1 1 SSR REVERSE 20 55.08 40 3 2 GAAGCAAAACGAAGATTGTC
CM001041.1 1 SSR FORWARD 20 55.81 45 2 0 GCATCACCTCTTCCCTATTT 255 5 2 GA 2 GAGAGAGAGAGAGA 14
CM001041.1 1 SSR REVERSE 20 55.37 40 5 2 ATTGCCTTGAAGATCAACAG
CM001041.1 1 SSR FORWARD 20 55.36 40 4 1 GATGAAACTCGAAGCATTGT 241 4 2 TC 2 TCTCTCTCTCTC 12
CM001041.1 1 SSR REVERSE 20 55.99 40 3 2 CATCAGTGGCAAAAATAAGC
CM001041.1 1 SSR FORWARD 20 55.07 40 6 0 GAGTTAACGGCTAAAGCAAA 221 5 0 AT 2 ATATATATATATATATATATATATATATATATATATATATATATATATATAT 52
CM001041.1 1 SSR REVERSE 21 55.11 42.86 3 2 CAACTCCCCTGACATAACATA
CM001041.1 1 SSR FORWARD 20 55.33 40 4 2 ACGAATAAGAAACGAGCGTA 246 6 0 AT 2 ATATATATATATATAT 16
CM001041.1 1 SSR REVERSE 20 55.22 45 4 2 CCTCTTTTCCACTACCATGA
CM001041.1 1 SSR FORWARD 20 55.12 40 4 2 ATGTTCTTTGAAGTCGGCTA 248 5 0 TA 2 TATATATATATA 12
CM001041.1 1 SSR REVERSE 20 55.99 40 7 0 TCAAATGTCAGTTTGGGTTC
CM001041.1 1 SSR FORWARD 20 55.3 40 5 3 AGGCACGTTAAACATAATGG 260 3 0 GT 2 GTGTGTGTGTGTGTGT 16
CM001041.1 1 SSR REVERSE 21 55.35 42.86 3 1 TCAATCATCAGAAGTCCAGAG
CM001041.1 1 SSR FORWARD 20 55.16 45 4 3 TGGCTAAAGTATCTGCCACT 242 7 2 AG 2 AGAGAGAGAGAGAGAGAG 18
CM001041.1 1 SSR REVERSE 20 55.29 50 4 2 CTCTGAAACTCTAGCGATGG
CM001041.1 1 SSR FORWARD 21 55.35 42.86 5 3 ATGGAAAGACGGCTATTAGAG 249 3 0 TC 2 TCTCTCTCTCTCTCTCTC 18
CM001041.1 1 SSR REVERSE 20 55.37 40 2 2 ACACCAAGACAAGAAAAACG
CM001041.1 1 SSR FORWARD 20 55.06 40 5 0 GGGTAAAGTTTGTGCTTTTG 269 4 0 GA 2 GAGAGAGAGAGA 12
CM001041.1 1 SSR REVERSE 20 55.2 40 4 0 AAGTCAACTGGTTTGTTTGG
CM001041.1 1 SSR FORWARD 20 55.2 50 0 0 GAAGGAAGAGGAGGAGAGAA 243 2 0 GA 2 GAGAGAGAGAGAGAGAGAGA 20
CM001041.1 1 SSR REVERSE 20 55.97 40 2 0 TTTTGGAGGATGTAGAAGCA
CM001041.1 1 SSR FORWARD 22 55.1 40.91 4 3 GGAGTCTGTTCAATCTCAACTT 249 7 0 TA 2 TATATATATATA 12
CM001041.1 1 SSR REVERSE 20 55.19 45 3 0 TACTGAACGACTTTGGCTCT
CM001041.1 1 SSR FORWARD 20 55.03 45 3 2 GCTTGACATTCTCCTTATGC 261 3 1 TA 2 TATATATATATATATA 16
CM001041.1 1 SSR REVERSE 20 55.03 45 4 2 GAGTTGGACATGGGTCTAAA
CM001041.1 1 SSR FORWARD 20 55.93 50 3 2 CAGTCAATCGTCCTACTTCG 265 3 0 AT 2 ATATATATATAT 12
CM001041.1 1 SSR REVERSE 20 56.03 50 6 3 CACGTGGCTACACTTAAAGG
CM001041.1 1 SSR FORWARD 20 55.31 45 2 0 GGGGGAAGATAGAAGATTTG 258 3 0 GT 2 GTGTGTGTGTGTGTGT 16
CM001041.1 1 SSR REVERSE 20 56.43 40 4 3 CGCACACCGAATAAGAATTA
CM001041.1 1 SSR FORWARD 19 55.18 47.37 3 1 GCTATTGAAGCCGAAACTC 272 3 1 AT 2 ATATATATATATAT 14
CM001041.1 1 SSR REVERSE 20 55.09 45 4 2 GGAGACGTTCTGTGAAATGT
CM001041.1 1 SSR FORWARD 20 55.19 45 4 2 CATCTCCTTCGACAACATCT 256 3 2 CA 2 CACACACACACA 12
CM001041.1 1 SSR REVERSE 20 55.02 45 6 0 TTCTGAGGTAGCACAGGTTT
CM001041.1 1 SSR FORWARD 20 55.52 50 4 2 CTCCATACGTTAGCGAGTGT 247 4 2 TC 2 TCTCTCTCTCTC 12
CM001041.1 1 SSR REVERSE 20 55.36 55 3 1 GAGATTAGGGACCGAGAGAC
CM001041.1 1 SSR FORWARD 20 55.06 45 4 3 ATAACATGCACCCAAGACTC 242 5 1 AT 2 ATATATATATAT 12
CM001041.1 1 SSR REVERSE 20 55.07 45 4 0 CAAAAAGCTCCATGTCTCTC

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