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Chromosome15 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001040.1 1 SSR FORWARD 20 55.29 45 3 2 TCCAACGAAGGTGTTTACTC 260 4 1 CT 2 CTCTCTCTCTCT 12
CM001040.1 1 SSR REVERSE 20 55.02 40 5 0 GAATGACCGTGATGAGTTTT
CM001040.1 1 SSR FORWARD 20 55.45 50 4 0 GTATAGCGACCTACCGTGAA 243 3 0 TA 2 TATATATATATATA 14
CM001040.1 1 SSR REVERSE 20 55.04 45 4 2 GCTCATCTGGATAGAAAACG
CM001040.1 1 SSR FORWARD 20 55.45 50 4 0 GTATAGCGACCTACCGTGAA 243 3 0 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 20 55.04 45 4 2 GCTCATCTGGATAGAAAACG
CM001040.1 1 SSR FORWARD 20 55.79 55 4 1 CTATACACGCTCTGGTGTCC 262 4 1 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 20 55.89 40 3 2 TCCGTTGGAAAGAACAAGTA
CM001040.1 1 SSR FORWARD 20 55.38 40 4 3 ATTCTGGATTCTCACCATGA 238 3 3 GA 2 GAGAGAGAGAGAGAGAGAGA 20
CM001040.1 1 SSR REVERSE 20 55.01 50 1 1 TCCTCTCACACACACTCTCA
CM001040.1 1 SSR FORWARD 19 55.91 47.37 4 2 GCGACTTGCGCTTTAGTAT 255 5 1 CT 2 CTCTCTCTCTCT 12
CM001040.1 1 SSR REVERSE 20 55.41 40 3 2 ACACTTCAAAATCGCCTATG
CM001040.1 1 SSR FORWARD 20 55.02 45 5 2 ACTCTTGACCTTGGCAACTA 258 3 2 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 46
CM001040.1 1 SSR REVERSE 20 55.1 45 4 3 CTTCTCACATGACCTTTGGT
CM001040.1 1 SSR FORWARD 21 55.1 47.62 4 1 GGGTTATGCCTAGAACTCTGT 275 5 1 AT 2 ATATATATATAT 12
CM001040.1 1 SSR REVERSE 20 55.02 45 4 2 AACACAGACACATGGTGATG
CM001040.1 1 SSR FORWARD 22 55.58 40.91 7 2 CTACAAGCCGTTTGTTTCTAGT 261 3 0 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 21 55.19 47.62 5 0 AAGAGTCTGACCAGTGTGATG
CM001040.1 1 SSR FORWARD 20 56.04 45 2 2 GATAAGGCAAAAGAGGTCGT 252 6 1 TC 2 TCTCTCTCTCTCTC 14
CM001040.1 1 SSR REVERSE 20 55.76 50 2 1 GCTTTTACTTCTCCCTCGTC
CM001040.1 1 SSR FORWARD 20 55.16 40 3 0 AAAGAAGTGAGTCCAAGCAA 244 6 3 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 55.37 40 3 3 CTTGCCATCTCAGTTCAAAT
CM001040.1 1 SSR FORWARD 20 55.82 50 6 2 GTTGAGACGGCTAGCTTGTA 254 4 1 GA 2 GAGAGAGAGAGA 12
CM001040.1 1 SSR REVERSE 20 55.19 45 2 0 TTACTTGTCCTTGCTCTCGT
CM001040.1 1 SSR FORWARD 20 55.24 45 3 3 GAAACTCAAAGTCCACAGGA 234 4 0 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 32
CM001040.1 1 SSR REVERSE 20 55.38 45 4 2 CGCCACTTCTCTACAAAAAG
CM001040.1 1 SSR FORWARD 20 55.94 40 6 3 CTTGGTCATGATTGTCGAAT 236 4 3 GA 2 GAGAGAGAGAGA 12
CM001040.1 1 SSR REVERSE 20 55.64 40 4 1 TTGGTCTCGATTCATTCACT
CM001040.1 1 SSR FORWARD 21 55.12 42.86 6 0 ACTTGTAAGTGTGGCTTGAGA 252 4 1 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 56.88 40 4 2 ACCCTTTTCAGCCCTTTAAT
CM001040.1 1 SSR FORWARD 21 55.12 42.86 6 0 ACTTGTAAGTGTGGCTTGAGA 252 4 1 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 56.88 40 4 2 ACCCTTTTCAGCCCTTTAAT
CM001040.1 1 SSR FORWARD 20 55.68 40 4 3 AGCTTTTCTTCACCCAAGTT 239 5 0 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGA 28
CM001040.1 1 SSR REVERSE 20 55.19 45 7 2 AGAAATCCAGGATCAGGTCT
CM001040.1 1 SSR FORWARD 20 55.39 40 4 1 GTTTCCATCATCATTTCTGG 260 4 0 TC 2 TCTCTCTCTCTC 12
CM001040.1 1 SSR REVERSE 20 55.29 40 3 2 AGATGTCAAAGGGGAAGTTT
CM001040.1 1 SSR FORWARD 20 55.68 40 4 3 AGCTTTTCTTCACCCAAGTT 239 5 0 GA 2 GAGAGAGAGAGAGAGAGAGAGAGAGAGA 28
CM001040.1 1 SSR REVERSE 20 55.19 45 7 2 AGAAATCCAGGATCAGGTCT
CM001040.1 1 SSR FORWARD 20 55.39 40 4 0 GTTTCCATCATCATTTCAGG 260 4 0 TC 2 TCTCTCTCTCTC 12
CM001040.1 1 SSR REVERSE 20 55.29 40 3 2 AGATGTCAAAGGGGAAGTTT
CM001040.1 1 SSR FORWARD 20 55.77 45 4 2 ATCGGGGATGCTATACTTTC 283 3 0 AT 2 ATATATATATATATAT 16
CM001040.1 1 SSR REVERSE 20 55.26 40 2 2 TCTGGTCTTTCCTTGCTTTA
CM001040.1 1 SSR FORWARD 20 55.38 50 2 0 CACCCTTTTCCTCTATCTCC 257 4 0 AG 2 AGAGAGAGAGAGAGAGAGAGAGAGAGAGAG 30
CM001040.1 1 SSR REVERSE 20 55.82 50 5 1 TCTCAGATCCGTAGTCTCCA
CM001040.1 1 SSR FORWARD 20 55.52 45 3 2 AAAGAGAGGGCAGTTTTAGC 268 5 2 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 20 55.57 45 2 0 AATCACCCTAAACCCAACTC
CM001040.1 1 SSR FORWARD 19 55.19 42.11 4 2 CTTTGATCGTTGTTTGACG 234 3 1 TC 2 TCTCTCTCTCTCTCTCTCTC 20
CM001040.1 1 SSR REVERSE 20 55.07 50 3 1 GATGTCGGAAGTGTCTCTGT
CM001040.1 1 SSR FORWARD 19 55.19 42.11 4 2 CTTTGATCGTTGTTTGACG 234 3 1 CT 2 CTCTCTCTCTCT 12
CM001040.1 1 SSR REVERSE 20 55.07 50 3 1 GATGTCGGAAGTGTCTCTGT
CM001040.1 1 SSR FORWARD 20 55.39 40 3 1 TGAAACTCTCCCCCTTTATT 250 4 0 AT 2 ATATATATATATATATAT 18
CM001040.1 1 SSR REVERSE 20 55.11 40 4 0 CTGATCCCCTTTTCTTTCTT
CM001040.1 1 SSR FORWARD 20 56.65 40 5 2 GCCCATTTTTCATCTGATCT 253 4 0 AG 2 AGAGAGAGAGAG 12
CM001040.1 1 SSR REVERSE 20 55.33 50 2 1 TCTCATCACTCCCTTCTCTG
CM001040.1 1 SSR FORWARD 20 55.52 40 5 2 CGGAATCTAATCATTCATGG 224 3 1 CT 2 CTCTCTCTCTCTCTCT 16
CM001040.1 1 SSR REVERSE 20 55.12 45 3 0 GATAGCGTTTCACTTTCAGG
CM001040.1 1 SSR FORWARD 20 55.29 40 3 2 CTCGTTTGACTGTTTCCAAT 269 4 1 AG 2 AGAGAGAGAGAGAGAG 16
CM001040.1 1 SSR REVERSE 20 55.04 40 2 1 AGATGAACGCAGGAAGATAA
CM001040.1 1 SSR FORWARD 20 55.12 40 2 2 TTTGGAACGAAGAGCAGTAT 252 5 1 AG 2 AGAGAGAGAGAGAGAGAG 18
CM001040.1 1 SSR REVERSE 20 55 40 5 1 GAAAGAGATTCGTCGATTTG
CM001040.1 1 SSR FORWARD 20 55.33 40 4 3 TTTCAGCAGAGTGATTGTCA 248 6 1 CT 2 CTCTCTCTCTCT 12
CM001040.1 1 SSR REVERSE 20 55.19 45 4 1 CAACAACTCTTCCACCTGTT
CM001040.1 1 SSR FORWARD 20 55.17 50 6 3 TGCAGCTGTAGAAGACAGTG 248 5 0 GT 2 GTGTGTGTGTGT 12
CM001040.1 1 SSR REVERSE 20 55.7 40 4 0 ACGAAAACAAGTGCAGAAAG
CM001040.1 1 SSR FORWARD 20 55.46 45 3 1 TAGGTTGCTCCTTGCTCTAA 245 4 1 AG 2 AGAGAGAGAGAG 12
CM001040.1 1 SSR REVERSE 20 55.18 45 4 1 ATGAGGAAACATGCAGAGAC
CM001040.1 1 SSR FORWARD 20 55.59 45 7 2 TTTCAGAACTTCTCCTCGTG 251 4 1 TA 2 TATATATATATATATATA 18
CM001040.1 1 SSR REVERSE 20 55.37 45 4 0 TCTGGGACTACTTTTGCATC
CM001040.1 1 SSR FORWARD 20 55.36 40 3 1 ATTCTCAATCACGCAACTTC 240 4 0 AC 2 ACACACACACAC 12
CM001040.1 1 SSR REVERSE 20 55.14 40 2 0 GCCTTTTTATTGTCCCTTCT
CM001040.1 1 SSR FORWARD 20 55.38 40 6 2 TTTAACTTGGCTTGATCACC 245 5 3 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 55.29 40 4 3 TCTTATGAGTGTCGCATCAA
CM001040.1 1 SSR FORWARD 20 55.32 45 3 2 GGTTCACACGGATTTGTATC 257 3 2 CT 2 CTCTCTCTCTCT 12
CM001040.1 1 SSR REVERSE 20 55.09 50 2 0 GTTAGAAGGAAGGAGGGAGA
CM001040.1 1 SSR FORWARD 20 55.84 40 6 0 CGGTTCGAATTTTAGTGATG 257 3 2 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 55.24 45 3 0 TTCTGTTTTCTCCTGACACC
CM001040.1 1 SSR FORWARD 20 55.22 45 3 1 CAAGCAAAAGGGTAAGTCTG 250 4 3 AT 2 ATATATATATATATAT 16
CM001040.1 1 SSR REVERSE 20 55.24 50 6 3 TCTTCCTCTCGTCGACTCTA
CM001040.1 1 SSR FORWARD 20 55.07 40 3 1 CTGAATAAAAGCCCATCAAG 251 5 2 AT 2 ATATATATATAT 12
CM001040.1 1 SSR REVERSE 20 55.17 40 8 2 GGAAACAAGTTTCAGATGGA
CM001040.1 1 SSR FORWARD 20 55.17 40 6 3 ATCACCTAGCCAAATGATTG 249 3 2 GT 2 GTGTGTGTGTGT 12
CM001040.1 1 SSR REVERSE 20 55.01 40 5 0 GAGCTTAGCCAAAAGAAACA
CM001040.1 1 SSR FORWARD 20 55.2 40 4 2 ATCCGTCTGTTTGAATTGAG 242 5 3 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 20 55 45 6 0 CGTCGCTAGCTCATATTTCT
CM001040.1 1 SSR FORWARD 20 55.2 40 4 2 ATCCGTCTGTTTGAATTGAG 250 4 1 AT 2 ATATATATATATATATATATATATATATAT 30
CM001040.1 1 SSR REVERSE 19 55.35 57.89 4 2 CCCCCACACTCTATACGTC
CM001040.1 1 SSR FORWARD 20 55.83 50 8 1 CCCTAGGGACCTAGAAATGA 246 3 1 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 55.21 45 2 2 ACTCACATCACTCACCCAAT
CM001040.1 1 SSR FORWARD 20 55.2 45 2 2 GGACGAGACCAAGATTACAA 269 4 3 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 20 55.25 45 4 2 GGACTGGTGCATACCTTTTA
CM001040.1 1 SSR FORWARD 20 55.35 40 4 3 CGGACGATTTTGGTCTATAA 254 5 1 TA 2 TATATATATATATATATATATATA 24
CM001040.1 1 SSR REVERSE 20 55.64 45 6 2 CTGAAGTCGACATGGAAATC
CM001040.1 1 SSR FORWARD 20 55.25 45 4 2 GGACTGGTGCATACCTTTTA 269 4 3 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 20 55.2 45 2 2 GGACGAGACCAAGATTACAA
CM001040.1 1 SSR FORWARD 20 56.88 45 5 0 CATCCTCGGACCTAAGAAAA 237 4 3 TA 2 TATATATATATATATA 16
CM001040.1 1 SSR REVERSE 20 57.61 40 5 3 CGTTTTTGGGATTTCCAGTA
CM001040.1 1 SSR FORWARD 19 55.65 52.63 4 2 GCCACTAGGAAACATCGAC 265 2 0 AT 2 ATATATATATATATATATATAT 22
CM001040.1 1 SSR REVERSE 20 55.64 45 6 3 GGGGTATACGCAGGAATAAT
CM001040.1 1 SSR FORWARD 20 55.13 45 2 0 TGGGTAGAAGGATGAAGATG 241 4 3 TG 2 TGTGTGTGTGTGTGTG 16
CM001040.1 1 SSR REVERSE 20 55.34 40 4 2 TAACCAACCCTCACATTGAT
CM001040.1 1 SSR FORWARD 20 55.58 45 7 0 GATTCAATCACCAGCGATAC 260 6 3 TC 2 TCTCTCTCTCTCTC 14
CM001040.1 1 SSR REVERSE 20 55.54 45 5 1 GGTATTGTTGGCACATTAGG
CM001040.1 1 SSR FORWARD 21 55.18 42.86 2 0 CACATTAGCATTAGGAGCAAC 275 3 1 TC 2 TCTCTCTCTCTCTC 14
CM001040.1 1 SSR REVERSE 21 55.03 42.86 4 1 TTGGAGAGAGAGCTTAGGATT
CM001040.1 1 SSR FORWARD 20 55.07 40 8 0 CATGTATGCATGAATTGCTC 250 5 3 TG 2 TGTGTGTGTGTGTGTGTG 18
CM001040.1 1 SSR REVERSE 20 55.32 40 5 2 AGATGCAATGCTTATCAACC
CM001040.1 1 SSR FORWARD 20 56.14 40 3 1 TTCCATTAGGTTTTCCTGGT 234 4 0 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 55.37 40 8 2 AGAAGAGGGAATCGATTGTT
CM001040.1 1 SSR FORWARD 20 55.49 50 4 0 TGGCGGTTACTAGACTTCAC 251 5 1 AC 2 ACACACACACACACACAC 18
CM001040.1 1 SSR REVERSE 20 55.05 40 5 0 TCTCTTTTCTGAAGGACCAA
CM001040.1 1 SSR FORWARD 20 55.49 50 4 0 TGGCGGTTACTAGACTTCAC 251 5 1 AT 2 ATATATATATATAT 14
CM001040.1 1 SSR REVERSE 20 55.05 40 5 0 TCTCTTTTCTGAAGGACCAA
CM001040.1 1 SSR FORWARD 20 55.11 40 2 0 CTCTTCTCTTCCCCATTTTT 237 5 1 TC 2 TCTCTCTCTCTCTC 14
CM001040.1 1 SSR REVERSE 20 55.03 40 3 2 AAAATCATCAGTCCCAAGTG
CM001040.1 1 SSR FORWARD 20 55.43 40 2 0 CACCAAATAAAACCAACACC 242 7 3 CA 2 CACACACACACA 12
CM001040.1 1 SSR REVERSE 20 55.39 40 5 3 GTTGATTTTCCAGGATGATG
CM001040.1 1 SSR FORWARD 20 55.43 40 2 0 CACCAAATAAAACCAACACC 242 7 3 AT 2 ATATATATATATATAT 16
CM001040.1 1 SSR REVERSE 20 55.39 40 5 3 GTTGATTTTCCAGGATGATG
CM001040.1 1 SSR FORWARD 18 57.52 50 2 0 ATAAAATCCCGCCCGTAG 260 3 0 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 55.84 45 6 2 ATCTGATGCCTCACGAGTAA
CM001040.1 1 SSR FORWARD 20 55.06 45 5 1 AGCCACAATGACATAAGGAC 241 7 1 AT 2 ATATATATATAT 12
CM001040.1 1 SSR REVERSE 20 55.37 40 7 3 CAAGTCGTTTGTCAAAACCT
CM001040.1 1 SSR FORWARD 20 55.19 45 3 3 GCAAGTTCAAGGCAAGTAAC 251 5 1 AT 2 ATATATATATAT 12
CM001040.1 1 SSR REVERSE 22 56.82 40.91 6 0 CGACTTGTTAAAAACACGAGAG
CM001040.1 1 SSR FORWARD 20 55.48 45 4 2 AAGCTGACCTCCAACCTAAT 262 4 0 TC 2 TCTCTCTCTCTCTC 14
CM001040.1 1 SSR REVERSE 20 55.1 45 2 0 ATTTACCTATCCCTCGCTTC
CM001040.1 1 SSR FORWARD 20 55.48 45 4 2 AAGCTGACCTCCAACCTAAT 262 4 0 TC 2 TCTCTCTCTCTCTC 14
CM001040.1 1 SSR REVERSE 20 55.1 45 2 0 ATTTACCTATCCCTCGCTTC
CM001040.1 1 SSR FORWARD 20 55.04 45 3 2 GTCTTGCCTAAGTGTTTTGG 254 3 2 GA 2 GAGAGAGAGAGA 12
CM001040.1 1 SSR REVERSE 20 55.66 50 4 2 GGGTCTAATCCCTCAACCTA
CM001040.1 1 SSR FORWARD 22 57.05 45.45 6 2 GGATTTCTATGGAAGTGCTAGG 300 4 3 TA 2 TATATATATATATA 14
CM001040.1 1 SSR REVERSE 22 56.32 40.91 4 3 TTGTGAAGCTAAGAGCCTAAGA
CM001040.1 1 SSR FORWARD 20 56.17 45 4 3 GAACTGAAGCTCCCCTTAAA 262 4 2 AT 2 ATATATATATATATATAT 18
CM001040.1 1 SSR REVERSE 20 55.3 40 6 0 TTTACCGGTGTTGTTTTCTC
CM001040.1 1 SSR FORWARD 20 55.76 45 4 2 TCGGAACCTTGTGTACCTTA 279 3 2 TA 2 TATATATATATATATATA 18
CM001040.1 1 SSR REVERSE 18 55.47 44.44 4 2 AAATTAGGAAGCGGAACG
CM001040.1 1 SSR FORWARD 20 55.3 45 2 2 CAACTAAAGAAGAGCGATGG 237 5 0 TA 2 TATATATATATA 12
CM001040.1 1 SSR REVERSE 20 55.12 40 3 1 AAGATTGGTTGGTCACTTTG
CM001040.1 1 SSR FORWARD 20 55.19 50 4 2 GAACTCACTCGGGTATGAGA 252 3 0 AG 2 AGAGAGAGAGAG 12
CM001040.1 1 SSR REVERSE 20 56.01 50 3 2 CAAAGAGACGACACCGTAAG
CM001040.1 1 SSR FORWARD 20 55.5 40 3 0 ACACACCAAAACAGGGTAAA 257 6 1 AT 2 ATATATATATAT 12
CM001040.1 1 SSR REVERSE 21 55.01 42.86 4 2 CTTCTTGAATGGTGAGTGAAG
CM001040.1 1 SSR FORWARD 20 55.78 45 6 3 ATAGGCCCATCCATTCTTAG 274 7 3 CA 2 CACACACACACA 12
CM001040.1 1 SSR REVERSE 20 55.03 45 3 3 GTTTTCTGGGATGGTGACTA
CM001040.1 1 SSR FORWARD 20 55.24 45 4 0 TTACCACTGCCTAGCTGTTT 250 4 2 TG 2 TGTGTGTGTGTG 12
CM001040.1 1 SSR REVERSE 19 55.53 57.89 5 1 GTTCCCAGAGTCTCCACAG
CM001040.1 1 SSR FORWARD 20 55.77 40 3 0 TCTTTGTGTGTGCGTAAATG 224 6 1 TA 2 TATATATATATATATATA 18
CM001040.1 1 SSR REVERSE 20 55.17 40 3 3 TGTCCACCAAGAAAAGAATC
CM001040.1 1 SSR FORWARD 19 55.2 42.11 8 2 TCCAGGAATAATCCTTTGC 253 3 0 GA 2 GAGAGAGAGAGA 12
CM001040.1 1 SSR REVERSE 20 55.5 55 2 0 CAATCCCCTCTCTCTCTCTC
CM001040.1 1 SSR FORWARD 20 55.09 45 5 1 GTCTGTGACAATCTCCGTTT 263 4 0 AG 2 AGAGAGAGAGAGAGAGAGAG 20
CM001040.1 1 SSR REVERSE 20 56.27 40 7 1 GGCCAAAAACTTTTACATCC
CM001040.1 1 SSR FORWARD 20 55.17 40 3 1 GAAGCACAAAAGGAAAGTTG 249 3 0 AG 2 AGAGAGAGAGAGAGAGAGAGAGAGAG 26
CM001040.1 1 SSR REVERSE 20 55.39 45 3 1 ATGACCACGAGAATCAGTGT
CM001040.1 1 SSR FORWARD 20 55.43 40 4 0 CTGAGTTTGAGGGAAAACAA 261 4 3 AT 2 ATATATATATATATATATATATATATATATAT 32
CM001040.1 1 SSR REVERSE 20 55.25 45 6 2 GTCCGCGTAAGTTAAGTTTG

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