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Chromosome14 primers

by | Sep 18, 2018 | Primers | 0 comments

Seq ID Count Primer type Orientation Len tm GC% Any compl 3′ compl Seq Prod size Pair any compl Pair 3′ compl Motif Motif Len SSR SSRLen
CM001039.1 1 SSR FORWARD 20 55.55 40 6 3 AAACCATGGAGAAGTTGACA 283 4 1 AT 2 ATATATATATATATAT 16
CM001039.1 1 SSR REVERSE 20 55.01 40 4 0 TGCTTACAAGTATCCCGTTT
CM001039.1 1 SSR FORWARD 20 55.55 40 6 3 AAACCATGGAGAAGTTGACA 283 4 1 AT 2 ATATATATATATATAT 16
CM001039.1 1 SSR REVERSE 20 55.01 40 4 0 TGCTTACAAGTATCCCGTTT
CM001039.1 1 SSR FORWARD 20 55.35 50 4 1 CGACAGTTCTCTCCACAGTT 248 4 1 AT 2 ATATATATATATATAT 16
CM001039.1 1 SSR REVERSE 20 56.66 45 5 3 GGAGGTTGTTACTTGCCAAT
CM001039.1 1 SSR FORWARD 20 55.58 40 7 2 GAAACGGCGTTAAGTAATGA 240 4 1 AT 2 ATATATATATATAT 14
CM001039.1 1 SSR REVERSE 20 55.15 45 4 3 TCTCTTTCACAGGTTGATCC
CM001039.1 1 SSR FORWARD 20 56.56 40 6 2 CGTGTGTTTAGCAAAAAGGA 256 6 3 AT 2 ATATATATATATATATATATAT 22
CM001039.1 1 SSR REVERSE 20 55.22 40 4 2 GTGGTTGGACTTTCGATAAA
CM001039.1 1 SSR FORWARD 21 55.07 42.86 5 3 ACATAACCGACAGTGTTGTTC 259 3 1 AT 2 ATATATATATATATATAT 18
CM001039.1 1 SSR REVERSE 20 55.8 45 6 2 ACCTAAGGCTGCAGAGAAAT
CM001039.1 1 SSR FORWARD 20 55.13 45 6 2 TCGAGCTCCATTCCTAGATA 251 4 0 TA 2 TATATATATATATATATATATATA 24
CM001039.1 1 SSR REVERSE 20 55.35 40 7 0 CGGTAATACCTCAATCGAAA
CM001039.1 1 SSR FORWARD 20 55.82 45 4 1 CTTCCAAAACCTGGGTAACT 274 3 1 AT 2 ATATATATATATAT 14
CM001039.1 1 SSR REVERSE 20 55.39 40 2 0 CCTTATCGGTCTCAACAAAA
CM001039.1 1 SSR FORWARD 20 56.28 45 6 2 AAGTCAGTCGTCGTGAATTG 256 5 1 TC 2 TCTCTCTCTCTC 12
CM001039.1 1 SSR REVERSE 20 55.2 40 5 1 AATTCGTCACAAGATTCCAG
CM001039.1 1 SSR FORWARD 20 55.97 45 6 2 GCAAAGGGGAAGTATTCCTA 252 4 1 CT 2 CTCTCTCTCTCTCT 14
CM001039.1 1 SSR REVERSE 20 55.52 40 4 2 CCAATTATCCAAGGTGTTTG
CM001039.1 1 SSR FORWARD 20 55.71 40 6 2 CCAAGTTTGAAAACGACATC 251 3 2 AT 2 ATATATATATATATATATATATATATATATATAT 34
CM001039.1 1 SSR REVERSE 20 55.38 50 4 2 AAGTCAGTCACCACCTGTGT
CM001039.1 1 SSR FORWARD 20 55.01 40 4 0 AAGAGCATATAGCCAATCCA 245 5 1 TA 2 TATATATATATATATA 16
CM001039.1 1 SSR REVERSE 20 55.6 40 4 2 AAATAAGGCTAAGGGCATGT
CM001039.1 1 SSR FORWARD 20 55.08 45 6 2 TCTGAGATCTAAAGCGAAGC 238 5 0 TC 2 TCTCTCTCTCTCTCTCTCTCTC 22
CM001039.1 1 SSR REVERSE 20 55.15 45 4 0 GATCTGGAACAAGGAGAACA
CM001039.1 1 SSR FORWARD 20 55.08 45 6 2 TCTGAGATCTAAAGCGAAGC 238 5 0 TC 2 TCTCTCTCTCTCTCTCTCTCTC 22
CM001039.1 1 SSR REVERSE 20 55.15 45 4 0 GATCTGGAACAAGGAGAACA
CM001039.1 1 SSR FORWARD 19 55.75 47.37 4 3 GAAAGTCGGCCCCTATTAT 223 3 3 AT 2 ATATATATATATATAT 16
CM001039.1 1 SSR REVERSE 20 55.51 40 3 1 AGAAAAGATTCCCTCCTTCA
CM001039.1 1 SSR FORWARD 20 55.95 45 6 2 CGAAAACGAGTGAGCTCTTA 234 5 2 AT 2 ATATATATATATAT 14
CM001039.1 1 SSR REVERSE 20 55.49 40 6 2 CTATTTTCACAGGCGTGTTT
CM001039.1 1 SSR FORWARD 20 55.12 40 4 2 ACTTTTCCAGAACCATGTTG 249 3 0 AT 2 ATATATATATATATAT 16
CM001039.1 1 SSR REVERSE 20 55.11 45 3 3 CTTTAGCCCCATCCTTAGAT
CM001039.1 1 SSR FORWARD 20 55.35 40 5 3 TCCCAGTTCTTGAATTTCTG 252 6 2 AC 2 ACACACACACACAC 14
CM001039.1 1 SSR REVERSE 20 55.07 40 8 3 TTCAAGCTTGGACTGATCTT
CM001039.1 1 SSR FORWARD 20 55.12 45 5 3 GGATGATCGGTTAAGTCTCA 250 3 1 AT 2 ATATATATATATATAT 16
CM001039.1 1 SSR REVERSE 20 55.3 40 5 1 GAGGCAATGTCCTTCAATAA
CM001039.1 1 SSR FORWARD 20 55.12 45 5 3 GGATGATCGGTTAAGTCTCA 250 3 1 TG 2 TGTGTGTGTGTG 12
CM001039.1 1 SSR REVERSE 20 55.3 40 5 1 GAGGCAATGTCCTTCAATAA
CM001039.1 1 SSR FORWARD 20 55.52 45 4 1 AACTCGGGTCTTCAGTTTCT 267 3 0 AT 2 ATATATATATATATAT 16
CM001039.1 1 SSR REVERSE 19 55.42 52.63 4 0 GGACGCCTGTGCTATACTT
CM001039.1 1 SSR FORWARD 20 55.43 45 6 2 TTAGGGACAGGGTCATGATA 241 3 0 AT 2 ATATATATATAT 12
CM001039.1 1 SSR REVERSE 20 55.51 40 3 2 TAAGCACACATCCACAAATG
CM001039.1 1 SSR FORWARD 21 55.53 42.86 5 0 AGCTGCTTTAGTGATTTCTCC 239 4 1 TG 2 TGTGTGTGTGTGTG 14
CM001039.1 1 SSR REVERSE 20 55.34 40 4 2 CATAAAAACGCCTTACAAGC
CM001039.1 1 SSR FORWARD 20 55.12 50 4 2 CTCCTTCTCCCTCGACTTAT 248 3 3 AC 2 ACACACACACACACAC 16
CM001039.1 1 SSR REVERSE 20 55.43 40 4 2 CCAATCCAAATATGAACGTC
CM001039.1 1 SSR FORWARD 20 55.02 40 6 0 TATTCCGGAGAGACTGAAAA 267 5 1 AG 2 AGAGAGAGAGAGAG 14
CM001039.1 1 SSR REVERSE 20 55.32 50 4 0 TACACGCTCCATATCTCCTC
CM001039.1 1 SSR FORWARD 20 55.45 40 4 2 CTCATTTTTACGAGCTTTGC 248 4 0 CT 2 CTCTCTCTCTCTCT 14
CM001039.1 1 SSR REVERSE 21 55.2 42.86 8 2 GAAACTGGTTTCCTCTTCCTA
CM001039.1 1 SSR FORWARD 20 55.15 45 4 0 TCACCCAACATCTTTCTCTC 268 3 0 TC 2 TCTCTCTCTCTCTCTC 16
CM001039.1 1 SSR REVERSE 20 55.23 40 4 2 CCCTTTCTGATTTACACCAA
CM001039.1 1 SSR FORWARD 20 55.02 45 4 3 TCCAACTAGCTTCACAAGGT 251 4 1 CA 2 CACACACACACA 12
CM001039.1 1 SSR REVERSE 20 55.06 40 5 3 TTGGTGTGTTCTGTCTGAAA
CM001039.1 1 SSR FORWARD 22 55.52 40.91 4 0 CTAGATTTTGATCCGTACAACC 224 4 0 AT 2 ATATATATATAT 12
CM001039.1 1 SSR REVERSE 20 57.34 45 7 0 TACCAAACCGTTTCCATACC
CM001039.1 1 SSR FORWARD 20 56.54 45 4 2 TAACAAAGAGGTGGGAGCTT 246 4 1 GA 2 GAGAGAGAGAGAGA 14
CM001039.1 1 SSR REVERSE 20 55.26 40 2 2 GCTGGGTTTGGTTTTATGTA
CM001039.1 1 SSR FORWARD 20 55.53 50 4 0 AGCTGACCAGTTGAGTCTTG 242 5 2 TC 2 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC 48
CM001039.1 1 SSR REVERSE 20 55.28 45 4 2 GCTAAAGACGAAAGACATGC
CM001039.1 1 SSR FORWARD 20 55.53 50 4 0 AGCTGACCAGTTGAGTCTTG 242 5 2 TC 2 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC 40
CM001039.1 1 SSR REVERSE 20 55.28 45 4 2 GCTAAAGACGAAAGACATGC
CM001039.1 1 SSR FORWARD 20 55.33 40 5 2 ACTTGCCCAAATAACAAGTG 256 3 0 TA 2 TATATATATATA 12
CM001039.1 1 SSR REVERSE 20 55.82 40 4 0 ATTCCCGAAGAAAATGGTAG
CM001039.1 1 SSR FORWARD 20 55.5 50 4 2 CACCACAGTCACAACGACTA 250 5 3 AC 2 ACACACACACACAC 14
CM001039.1 1 SSR REVERSE 20 55.25 50 4 2 GATGGAGTGTTGCAGAGAGT
CM001039.1 1 SSR FORWARD 20 55.85 55 4 2 AGGTAAGAGTCCACGTAGGG 258 5 2 CT 2 CTCTCTCTCTCTCTCTCT 18
CM001039.1 1 SSR REVERSE 20 55.4 45 3 0 ATTGTGGCTCCCTCTATCTT
CM001039.1 1 SSR FORWARD 18 55.92 44.44 4 0 TTCAATTAGGTGCGCTTG 254 4 1 TA 2 TATATATATATATATA 16
CM001039.1 1 SSR REVERSE 21 55.59 47.62 2 0 CCTTTTGATGTCTCTCTCTCC
CM001039.1 1 SSR FORWARD 20 55.04 40 4 2 ACAGAAAAGTGGGTTTGCTA 288 4 2 CT 2 CTCTCTCTCTCTCTCTCT 18
CM001039.1 1 SSR REVERSE 20 55.11 45 5 3 ATATCTGAAGGGGCTTTAGG
CM001039.1 1 SSR FORWARD 20 55.82 45 6 0 CCCCTTCAGATATCGTTCTT 251 4 1 GA 2 GAGAGAGAGAGA 12
CM001039.1 1 SSR REVERSE 20 55 40 5 3 AAACCCACATCAGATAATCG
CM001039.1 1 SSR FORWARD 20 55.17 40 4 2 CGTACAAAGGTTGGTTTCAT 246 4 0 AT 2 ATATATATATAT 12
CM001039.1 1 SSR REVERSE 20 55.15 45 3 0 TCATTGTTGACCTCTTCTCC
CM001039.1 1 SSR FORWARD 20 55.4 40 6 0 ACTTTGCGATCGATTAACAC 250 7 2 TG 2 TGTGTGTGTGTG 12
CM001039.1 1 SSR REVERSE 22 56.15 40.91 4 2 TTAATCGTACCTTGTTCTACCG
CM001039.1 1 SSR FORWARD 20 55.94 40 6 2 TTCCAGCGTTAACAACGTAT 265 7 0 TA 2 TATATATATATATATA 16
CM001039.1 1 SSR REVERSE 20 55.33 40 4 2 ATTAAAACGACCCTGGAAGT
CM001039.1 1 SSR FORWARD 20 56.27 40 6 2 CCGTGGCACAAATTATAAGA 264 5 2 TA 2 TATATATATATA 12
CM001039.1 1 SSR REVERSE 20 55.23 40 6 0 ATGACTTCATTGGTGGTGTT
CM001039.1 1 SSR FORWARD 20 55.45 45 4 2 TGTTCCTTAGCACAAGGTTC 248 4 2 TC 2 TCTCTCTCTCTC 12
CM001039.1 1 SSR REVERSE 20 55.02 50 4 2 GTTTGGGAGCTGTGTAGAAG
CM001039.1 1 SSR FORWARD 20 55.19 50 4 0 CTCTCTTAACCAACGCTCAC 236 3 0 CA 2 CACACACACACACACACACACACACACACA 30
CM001039.1 1 SSR REVERSE 20 55.07 40 8 0 CATGTATGCATGAATTGCTC
CM001039.1 1 SSR FORWARD 20 55.03 45 6 1 GATCGATGGAGATTACCTGA 236 4 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGA 24
CM001039.1 1 SSR REVERSE 20 55.94 45 3 3 TGTAACGGTGGGCTTTAGTA
CM001039.1 1 SSR FORWARD 20 55.06 50 4 0 TCTGCCGTACCTACTCTTGT 283 5 2 CT 2 CTCTCTCTCTCTCT 14
CM001039.1 1 SSR REVERSE 20 55.46 45 6 2 CACGTGTCAATACGTACCAA
CM001039.1 1 SSR FORWARD 23 55.25 43.48 6 0 TCAGTACTTGTCTACCAGTTTCC 232 5 3 AT 2 ATATATATATATATATATATAT 22
CM001039.1 1 SSR REVERSE 20 55.05 40 2 2 GGGGAACAACAAGAAACATA
CM001039.1 1 SSR FORWARD 20 56.15 40 5 3 GTCCACTTCGATTCAAACAA 264 3 0 AT 2 ATATATATATATATATATATAT 22
CM001039.1 1 SSR REVERSE 20 55.05 40 2 2 GGGGAACAACAAGAAACATA
CM001039.1 1 SSR FORWARD 23 55.25 43.48 6 0 TCAGTACTTGTCTACCAGTTTCC 228 5 3 AT 2 ATATATATATATATATATATAT 22
CM001039.1 1 SSR REVERSE 20 55.05 40 2 2 GGGGAACAACAAGAAACATA
CM001039.1 1 SSR FORWARD 20 55.03 45 6 1 GATCGATGGAGATTACCTGA 236 4 2 GA 2 GAGAGAGAGAGAGAGAGAGAGAGA 24
CM001039.1 1 SSR REVERSE 20 55.94 45 3 3 TGTAACGGTGGGCTTTAGTA
CM001039.1 1 SSR FORWARD 20 55.06 50 4 0 TCTGCCGTACCTACTCTTGT 283 5 2 CT 2 CTCTCTCTCTCTCT 14
CM001039.1 1 SSR REVERSE 20 55.46 45 6 2 CACGTGTCAATACGTACCAA
CM001039.1 1 SSR FORWARD 23 55.25 43.48 6 0 TCAGTACTTGTCTACCAGTTTCC 232 5 3 AT 2 ATATATATATATATATATATAT 22
CM001039.1 1 SSR REVERSE 20 55.05 40 2 2 GGGGAACAACAAGAAACATA
CM001039.1 1 SSR FORWARD 20 56.15 40 5 3 GTCCACTTCGATTCAAACAA 264 3 0 AT 2 ATATATATATATATATATATAT 22
CM001039.1 1 SSR REVERSE 20 55.05 40 2 2 GGGGAACAACAAGAAACATA
CM001039.1 1 SSR FORWARD 23 55.25 43.48 6 0 TCAGTACTTGTCTACCAGTTTCC 228 5 3 AT 2 ATATATATATATATATATATAT 22
CM001039.1 1 SSR REVERSE 20 55.05 40 2 2 GGGGAACAACAAGAAACATA
CM001039.1 1 SSR FORWARD 20 55.49 45 4 0 GTGTATCCAATGCAACCTTC 258 5 2 CT 2 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 38
CM001039.1 1 SSR REVERSE 20 55.32 45 4 2 CAGCACTTGAGGTTAATGGT
CM001039.1 1 SSR FORWARD 20 55.38 40 4 0 GGGATCAGCTAAACAAACAA 257 5 0 TA 2 TATATATATATATA 14
CM001039.1 1 SSR REVERSE 20 55.4 40 7 0 TATATCCGATACCCAAACCA
CM001039.1 1 SSR FORWARD 20 55.38 40 4 0 GGGATCAGCTAAACAAACAA 257 5 0 TA 2 TATATATATATATA 14
CM001039.1 1 SSR REVERSE 20 55.4 40 7 0 TATATCCGATACCCAAACCA
CM001039.1 1 SSR FORWARD 20 55.41 40 3 2 CGATTCAGTTGCTTGGTTAT 210 6 2 AT 2 ATATATATATATATATATATATATAT 26
CM001039.1 1 SSR REVERSE 19 55.83 42.11 4 2 ACAAACCAACGACATTTCC
CM001039.1 1 SSR FORWARD 20 55.51 40 4 0 TCTATGCGGTTAAGATTTGG 239 3 1 AT 2 ATATATATATAT 12
CM001039.1 1 SSR REVERSE 19 55.25 47.37 5 0 ATGATTCAGCTCCGTTCTC
CM001039.1 1 SSR FORWARD 20 55.63 45 3 0 GGTGTTTGAAGAAGTGGTTG 245 4 1 GA 2 GAGAGAGAGAGAGAGA 16
CM001039.1 1 SSR REVERSE 20 55.56 45 3 3 ACAACGAAGCACGAAGTTAC
CM001039.1 1 SSR FORWARD 20 55.1 45 4 0 TCTCCACGTAAGACAACACA 276 6 3 AG 2 AGAGAGAGAGAGAG 14
CM001039.1 1 SSR REVERSE 20 55.75 40 2 2 GTTGTTGTGGTTGGTTTGAT

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