Physicochemical properties of four phosphate solubilizing bacteria isolated from the semi-arid regions of North Gujarat, India
Physicochemical properties of phosphate solubilizing bacteria
DOI:
https://doi.org/10.21921/jas.v9i03.11008Keywords:
Physicochemical properties, phosphate solubilizing bacteria, North GujaratAbstract
The rhizosphere of several plants was screened for phosphate solubilizing bacteria (PSB)
from eight locations of North Gujrat. Thirty-three PSB were isolated in the study. Four PSB
isolates with the highest phosphate solubilization index between 1.55–2 were characterized
morphologically and biochemically and identified through 16S rRNA sequencing as
Klebsiella aerogenes, Klebsiella pneumoniae, Kocuria flava and Enterobacter hormaechei.
The growth of isolates was measured by plotting an optical density-based semi-logarithmic
growth curve. The isolates were measured for P solubilization in Pikovskaya broth. The
isolate K. flava LC515414 solubilized maximum Ca 3 PO 4 (7.63 µm P mL -1 ). The acid and
alkaline phosphatase activity of the isolates was also measured for 96 h in the growth
medium. After 96 h of growth, the isolate K. flava LC515414 had the highest final acid and
alkaline phosphatase activity of 4.68 U L -1 and 5.67 U L -1 .
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